1 1 Division of Administrative Hearings 2 Department of Administration, State of Florida 3 SUGAR CANE GROWERS COOPERATIVE ) of FLORIDA; ROTH FARMS, INC.; and, ) 4 WEDGEWORTH FARMS, INC., ) Petitioners, ) 5 V ) DOAH SOUTH FLORIDA WATER MANAGEMENT ) Case 92-3038 6 DISTRICT, an agency of the State ) of Florida; et al., ) 7 Respondents. ) FLORIDA SUGAR CANE LEAGUE, INC.; ) 8 UNITED STATES SUGAR CORPORATION; ) and NEW HOPE SOUTH, INC., ) 9 Petitioners, ) V ) DOAH 10 SOUTH FLORIDA WATER MANAGEMENT ) Case 92-3039 DISTRICT, an agency of the State ) 11 of Florida; et al., ) Respondents. ) 12 FLORIDA FRUIT and VEGETABLE ) ASSOCIATION; LEWIS POPE FARMS; ) 13 W.E. SCHLECHTER & SONS, INC., ) and HUNDLEY FARMS, INC., ) 14 Petitioners, ) V ) DOAH 15 SOUTH FLORIDA WATER MANAGEMENT ) Case 92-3040 DISTRICT, an agency of the State ) 16 of Florida; et al., ) Respondents. ) 17 Volume I 18 Deposition of David Swift 19 Taken before April Y. Sapp, Court Reporter and Notary Public in and for the State of Florida at 20 large, pursuant to notice of taking deposition filed by the Petitioners in the above cause. 21 - - - Monday December 14, 1992 22 319 Clematis Street West Palm Beach, Florida 33401 23 9:15 - 12:35 p.m. - - - 2 1 APPEARANCES: 2 On behalf of the Petitioners Florida Sugar 3 Cane League, Inc., United State Sugar Corp., and New South Hope, Inc.: 4 Peeples, Earl & Blank, P.A. 215 South Monroe Street 5 Suite 350 Tallahassee, Florida 32301 6 By: WILLIAM L. HYDE, ESQUIRE 7 On behalf of the Respondent SFWMD: South Florida Water Management District 8 3301 Gun Club Road West Palm Beach, Florida 33406 9 By: RUTH CLEMENTS, ESQUIRE 10 On behalf of the Intervenor United States of America: Assistant United States Attorney 11 Southern District of Florida 155 South Miami Avenue, Suite 627 12 Miami, Florida 33130-1693 By: THOMAS A.W. FITZGERALD, ESQUIRE 13 On behalf of Sugar Cane Growers: 14 Hopping, Boyd, Green & Sams 123 South Calhoun Street 15 Tallahassee, Florida 32301 By: GARY V. PERKO, ESQUIRE 16 Also Present: James Grimshaw 17 Miles M. (Bud) Smart 18 - - - 3 1 - - - 2 I N D E X 3 - - - 4 5 WITNESS: DIRECT CROSS REDIRECT RECROSS 6 David Swift 7 BY MR. HYDE 5 8 4 1 2 - - - 3 E X H I B I T S 4 - - - 5 6 NUMBER PAGE 7 EXB. NO. 1 8 8 Resume of David Swift 9 EXB. NO. 2 24 10 Technical Publication 81-5 11 EXB. NO. 3 83 12 Technical Publication 87-2 13 EXB. NO. 4 85 14 Periphyton document, 11-15-91 15 EXB. NO. 5 94 16 Ecological Indicators of Water Quality 17 in the Wetlands of S. Florida 18 EXB. NO. 6 97 19 David Swift 1987 Abstract 5 1 P R O C E E D I N G S 2 - - - 3 Thereupon, 4 David Swift, 5 being by the undersigned Notary Public first duly 6 sworn, was examined and testified as follows: 7 THE WITNESS: I do. 8 DIRECT (David Swift) 9 BY MR. HYDE: 10 Q. For purposes of the record my name is 11 William Hyde. I'm representing the Florida Sugar 12 Cane League, Inc., United States Sugar Corporation 13 and New Hope South in proceedings on the Water 14 Management District's Everglades SWIM Plan. 15 First of all, let me ask you, is it 16 Dr. Swift? 17 A. No. 18 Q. Mr. Swift, have you ever been in a 19 deposition before? 20 A. Yes, sir. 21 Q. Okay. So you pretty much know the drill 22 about how they are performed? 23 A. Yes, sir. 24 Q. Okay. I would like you to note that if I 25 ask any questions that you don't understand, or if 6 1 they are confusing to you in any regard, please tell 2 me so and I'll try to redraft the question. 3 Otherwise, I will presume, for purposes of this 4 record, that you understand the question and you're 5 trying to be responsive to it. 6 I would also note that if any of the 7 attorneys in this room object at any time I would 8 suggest that you just stop what you're saying at that 9 point and allow the attorneys to flesh out that 10 objection. Usually it will allow you to answer the 11 question anyway, but there may be some rare 12 circumstances where your attorney in particular will 13 instruct you not to answer. 14 Mr. Swift, where are you currently 15 employed? 16 A. South Florida Water Management District, 17 West Palm Beach. 18 Q. Okay. And in what capacity are you 19 employed there? 20 A. My title is senior scientific -- I get 21 confused. We change this every year. Senior 22 Environmentalist is the title that I recall. 23 Q. And what duties and responsibilities does 24 that position entail? 25 A. Well, it varies from division to division. 7 1 Currently I'm project manager, for example, on the 2 Lake Okeechobee SWIM Plan. 3 Q. Okay. Does it have any other 4 responsibilities at this point? 5 A. The position or my -- 6 Q. Your particular role in that position as a 7 Senior Environmentalist. 8 A. Pretty much as project manager, although 9 there are other Senior Environmentalists that are not 10 project managers, obviously. 11 Q. I'd like to make an initial inquiry into 12 your educational background and I'd like you to start 13 first with your college degree. 14 Could you tell me where, when and in what 15 field you obtained that degree? 16 A. My undergraduate degree was from Adrian 17 College in Adrian, Michigan. Bachelor of Science 18 degree in zoology. 19 Q. When did you obtain that degree? 20 A. 1968. 21 Q. Did you do any undergraduate thesis in that 22 regard? 23 A. No. 24 Q. Did you obtain any honors in that position? 25 A. I don't believe so, other than belonging to 8 1 a biological club so to speak. You have to have a 2 certain grade point. 3 MS. CLEMENTS: Bill, we have a copy of the 4 cv. That might assist you in this. 5 MR. HYDE: It might. 6 BY MR. HYDE: 7 Q. So you were employed by ESE at one point. 8 MR. HYDE: Why don't we go ahead and attach 9 this resume to the deposition as Exhibit 1, is 10 that okay? 11 (The document was marked 12 Exb. No. 1.) 13 BY MR. HYDE: 14 Q. Mr. Swift, I'd like you to identify the 15 document that's labeled Exhibit 1. 16 A. Exhibit 1 is a resume. 17 Q. Okay. 18 A. My resume. 19 Q. Okay. Is the information contained in that 20 resume a true and accurate reflection of your 21 educational and work related experience? 22 A. Yes, sir. 23 Q. Okay. Let's go back now to your 24 educational background. Your resume reflects that 25 you obtained a Master of Science degree in aquatic 9 1 biology from Eastern Michigan University, is that 2 correct? 3 A. Correct. 4 Q. Could you tell me what your Master's thesis 5 in that regard was? 6 A. It was called, A Preliminary Study of 7 Diatoms of South Lake Michigan, January through 8 October 1972. 9 Q. What was the purpose of that study? 10 A. It was a floristic study of a freshwater 11 lake and what Eastern Michigan University does is it 12 develops a department to develop an expertise for 13 every student. For example, if you get a Master of 14 Science degree out of Eastern, you're required to be 15 a specialist in a particular area. Mine was in 16 aquatic biology with a specialty in phycology. Other 17 people, for example, would graduate with a specialty 18 in invertebrate taxonomy; for example, systems 19 ecology or fisheries research, those kinds of things. 20 Q. What do you mean by the phrase, "a 21 floristic study"? 22 A. It's a study of the species of algae that 23 were present in the lake. 24 Q. Okay. What was the nature of some of the 25 courses that you took in that Master's program, if 10 1 you could give me a notion of what kind of courses 2 were taken by you in order to obtain that specialized 3 post graduate degree? 4 A. There -- first of all, there was a general 5 phycology course that I took that was kind of a 6 survey of algae. There is a survey of the -- I think 7 aquatic vascular plants. There's an ichthyology 8 course. There was a course in statistics. There was 9 a course in -- I believe I took biochemistry and 10 entomology, environmental chemistry. There was 11 several special courses, I guess. One was 12 evolutionary biology. I'm starting to get foggy now. 13 Q. I'm just trying to get a general picture of 14 what it is. 15 A. Sure. I received a National Science 16 Foundation grant in the summer of -- I think it was 17 1971, I believe. Maybe 1970 for Central Michigan 18 University and that was a summer course, basically 19 again, in phycology and limnology and invertebrate 20 studies at Beaver Island, which is a biological 21 station for Central Michigan University. 22 Q. Did you say vertebrate or invertebrate? 23 A. Invertebrate. 24 Q. When was your -- what was your first 25 position once you obtained your post graduate degree? 11 1 A. It was -- a fellow by the name of Hugh 2 Putnam called me in Michigan. I had been working as 3 a consultant when I was in graduate school for eco 4 something. I have forgotten. There was an Ann Arbor 5 consultant company doing -- I don't think it's on 6 there, as a matter of fact. They were doing a study 7 of the effect of outboard motors on northern lakes 8 and they were shipping samples to me and I was making 9 a little money on the side as far as a graduate 10 student. Anyways, they liked my work. There was a 11 southern company called Environmental Science 12 Engineering who was doing a southern study in 13 Gainesville looking at the effects of outboard motors 14 on Florida lakes basically. They had an EPA grant 15 and they asked if I would be interested in working 16 for Environmental Science and Engineering and, yes, I 17 said yes and moved to Gainesville, basically. 18 Q. Your resume reflects you worked from 1972 19 to 1975. Is the job summary that's reflected on your 20 resume a fair summation of the kinds of work you did 21 for ESE during that time? 22 A. Yeah. It was some project management. A 23 lot of it, though, was basically based on taxonomy of 24 algae and doing biological assessment of various 25 projects. I was the -- ESE's expert, I guess, in 12 1 algal studies, basically. 2 Q. Why did you leave the employ of ESE? 3 A. ESE had several I guess what you would call 4 purges. I notice that this gentleman is from 5 Breedlove & Associates. I was hired by Hugh Putnam, 6 Mr. Breedlove I think worked for Biotest I think at 7 the time from Chicago. Anyways, ESE was primarily 12 8 professors from the university and they had a very 9 small operation and they were expanding rapidly and 10 Biotest bought them out. I was basically brought in 11 to take Dr. Putnam's place. I liked working with 12 Hugh. He was a very good limnologist and he went to 13 Water and Air Research and he asked if I would be 14 interested in moving over to Water and Air Research. 15 Q. Water and Air Research is also located in 16 Gainesville? 17 A. Right. On Archer Road. Same kinds of 18 responsibilities; almost exact same job, actually. 19 Q. Okay. So would the job summary that's 20 reflected on your resume for your period of time at 21 WAR, Inc. be reasonably accurate? 22 A. Right. I had done a number of 23 environmental assessments, that sort of thing. I 24 actually got my job at the District through that 25 company. I was doing consulting work for South 13 1 Florida on Lake Okeechobee. That's how they were 2 interested in bringing me down to South Florida Water 3 Management District. 4 Q. Okay. In 1977 you left WAR and moved to 5 the South Florida Water Management District. Why did 6 you leave that earlier position? 7 A. Water and Air Research? 8 Q. Right. 9 A. I believe that -- I believe I could not see 10 missing -- I would get too much farther in that 11 particular organization. It was, again, in 12 Gainesville. A lot of consulting companies 13 originally start out with 10 or 12 professors who are -- 14 basically it was their tax write off kind of thing 15 and they were not a very aggressive company. They 16 were not expanding and I didn't see a long term 17 future there. I was kind of interested in looking at 18 some different, I guess, types of work, I think, in 19 South Florida and I interviewed for that job. 20 Q. Okay. In 1977 your resume reflects that 21 you moved to the South Florida Water Management 22 District in the capacity as a Research 23 Environmentalist. 24 Is the job summary set forth in your resume 25 a fair description of what your duties were -- 14 1 A. I believe so. 2 Q. -- at that time? 3 First category indicates you were project 4 manager of water quality/periphyton bio-monitoring 5 research programs in the Everglades Water 6 Conservation Areas at Lake Okeechobee, that you were 7 a technical writer for District environmental/water 8 quality assessment studies and the District expert in 9 the taxonomy and ecology of periphyton and 10 phytoplankton communities in the Florida Everglades 11 and Lake Okeechobee. 12 Did you have any other responsibilities 13 beyond those that I just described? 14 A. None that I can think of. 15 Q. Okay. Your resume reflects that you were a 16 research environmentalist for the District from 1977 17 to 1988. Did -- at any time during that period did 18 you obtain promotions or the like that would reflect 19 a higher status within the organization? 20 A. I think in -- it's hard for me to answer 21 because we redefined what an environmental scientist 22 is during that period of time, so I can't remember if 23 I became a Senior Environmentalist in 1988 or not. 24 Q. Okay. Actually -- 25 A. Close to that. 15 1 Q. I was asking you about what happened 2 between 1977 and 1988. Were there any promotions 3 during that time? 4 A. Yeah. I think I came in as a 2. I think I 5 was promoted to a Environmentalist 3 and those 6 categories don't exist any more, but that's what I'm 7 trying to say is that we had an Environmentalist 8 Entry Level 1, then they had a 2 and 3. I think I 9 came in as a 2 and I was promoted to a 3. There was 10 a 4th category, but before I had a chance even to get 11 that they changed the whole job description of 12 environmentalist, basically. 13 Q. Was there any significance in that 14 categorization other than say a salary difference 15 between Category 1, 2, 3? 16 A. I think there's more responsibility as far 17 as project management, I believe, is what it was 18 about. There are also some experience, I think, 19 experience factors; being there five years, that sort 20 of thing. 21 Q. Were you a project manager as a Category 2 22 project manager? 23 A. Yes. 24 Q. What's the difference between Category 1, 25 Category 2 or Category 3? 16 1 A. Hard to tell. I think as far as 2 Environmental Scientist 2 or Environmental Scientist 3 3, roughly I remember that it was something about 4 experience. I think having so many years of 5 experience at the District, I believe. There are 6 probably some other things, but I honestly can't 7 remember. 8 Q. Okay. Your resume reflects that from 1989 9 to 1992 you've been Senior Environmental Scientist 10 and Technical Editor for the Planning Department of 11 the District and it further reflects what kinds of 12 work you've done during that time. Again, is that a 13 fair reflection -- 14 A. Uh huh. 15 Q. -- of your work responsibilities during 16 that period of time? 17 A. Yes. 18 Q. Who currently do you answer to in the 19 hierarchy of the Water Management District? Who's 20 your supervisor? 21 A. My supervisor is a gentleman by the name of 22 Terry Clark. He's my direct supervisor. He's a 23 supervising professional. 24 Q. Okay. What's the program that he's in 25 charge of? 17 1 A. Um, he's a supervisor within what we call 2 Upper District Planning Division. 3 Q. Okay. What is the Upper District Planning 4 Division? Is that a geographic locale -- 5 A. Yes. 6 Q. -- or does that have some other 7 significance? 8 A. Yes. It's -- the Upper District Planning 9 really is a misnomer. What it means is it would 10 include Lake Okeechobee, the Kissimmee and the Lower 11 West Coast of Florida. It would not include what we 12 call the Lower East Coast which would be the Water 13 Conservation Areas, Everglades National Park, Florida 14 Keys. 15 Q. Okay. 16 A. So it is geographical. 17 Q. It seems that a lot of your work, at least 18 in the context of these proceedings, though, relates 19 to the Lower East Coast, yet you're in the Upper 20 Planning District. Is there any explanation for that 21 seeming discrepancy? 22 A. I don't think it's a discrepancy. I had a 23 choice I think a couple of years ago to do some 24 different things, basically. I had worked in 25 Everglades I think almost 12 years or 13 years, 18 1 worked with it, so to speak. I was interested in 2 some other issues. Lower West Coast, for example, is 3 an area that has not had a whole bunch of attention 4 as far as water management and it has a number of 5 problems. It's interesting from the fact that, you 6 know, it's not full of canals and has a lot of 7 pristine areas left. I was kind of interested in 8 being involved in development of water management 9 plans for Corkscrew Swamp, Big Cypress Basin, those 10 kinds of things. 11 Q. Do you currently have any responsibilities 12 or ongoing responsibilities for the Everglades SWIM 13 planning process other than to serve as an expert 14 witness in these proceedings? 15 A. No. 16 Q. Do you have any ongoing studies that relate 17 to the purpose of these Everglades SWIM Plan 18 proceedings? 19 A. No. 20 Q. So all of the work upon which you're going 21 to base your opinions has been done -- 22 A. Yes. 23 Q. -- is that a fair statement? 24 My notes reflect that you've been 25 designated by the Water Management District to 19 1 testify regarding the following subject matters: 2 Periphyton taxonomy and community structure; changes 3 in and causes of change in periphyton communities in 4 the Everglades; impact of nutrients on periphyton; 5 assessment of various scientific studies relating to 6 water quality and algal species and eutrophic algal 7 species in the Everglades Protection Area. 8 Is that a fair summation of the opinions or 9 testimony you'll be giving in this proceeding? 10 A. Yes. 11 Q. I'd like you to take me through each of 12 those general subject matters. 13 The first is periphyton taxonomy and 14 community structure. What do you -- what's going to 15 be the substance of that testimony? 16 A. Don't quite understand the question. If 17 you could -- 18 Q. Well, have you spoken to your attorneys 19 about the kind of testimony they expect you to offer 20 during this proceeding? 21 A. No. 22 Q. So you don't have any idea what they meant 23 when they said you would be testifying regarding 24 periphyton taxonomy and community structure? 25 A. Not specifically as far as is there a 20 1 specific amount of information that we would like to 2 present to you. I think I'm here to basically answer 3 questions on the documents that have been produced by 4 the District over the years. 5 (Discussion held off the record.) 6 MR. HYDE: Tom, is that your understanding 7 as to what the witnesses are supposed to be 8 testifying to in terms of expressing opinions 9 that they are going go offer at a final hearing? 10 MR. FITZGERALD: Well, on the record, the 11 reason I asked to go back on the record is that 12 you're touching on a matter that may affect the 13 course of discovery for many witnesses, not 14 solely this one, so I can't answer that question 15 in the way that would make any sense to anybody 16 reading this record unless you put on the record 17 your long statement to the witness and to 18 counsel for the District because that's the 19 predicate for your question. Then I'll try and 20 answer it. 21 MR. HYDE: Fair enough. Let me see if I 22 remember all I said. 23 There has been an ongoing issue or problem 24 as to the opinions that witnesses are to be 25 offering during deposition and that they would 21 1 intend to offer during a final hearing in this 2 matter. 3 It has been my understanding that the 4 witnesses were expected to be able to, in 5 effect, designate the -- or identify the 6 opinions that they would be testifying on and 7 the factual bases for those opinions and, in 8 fact, I think the U.S. has -- that is the United 9 States Attorney's Office has itself suggested 10 that this was the appropriate way in which to 11 conduct discovery and that witnesses should be 12 ready to testify as to specific opinions and 13 factual bases for those opinions and not just 14 simply come in and say, well ask me any 15 questions you want about my work and I'll tell 16 you the answers to them. 17 Tom, is that a fair reflection of your 18 understanding of what witnesses are to be doing 19 in the course of these depositions? 20 MR. FITZGERALD: I don't think I can agree 21 with the characterization. I think the problem 22 that you alluded to off the record earlier in 23 which you've sort of recited now regarding an 24 earlier question in a deposition which was not 25 satisfactory to the United States was based, in 22 1 part, as well on the fact that no final opinions 2 had been formed by that witness. 3 As I understand this witness' position, the 4 witness has stated that he's prepared to answer 5 questions on any of his work and presumably, if 6 you direct the questions to the witness in the 7 areas of designated testimony, he would give you 8 opinions. 9 I don't think it's proper for someone 10 necessarily to walk into a deposition and ask 11 for a narrative summary of the life and times of 12 a witness, certainly their professional life and 13 times and that's what you tried to do. 14 So I think the witness was correct. You 15 would never be permitted to elicit a narrative 16 summary of everything a witness might or might 17 not know at a hearing and it presents an 18 impossible burden for a witness who is himself 19 not schooled in the intricacies of the case to 20 necessarily render for you on the basis of a 21 generalized description of their expert 22 testimony everything they're going to testify 23 to. 24 MR. HYDE: Okay. 25 MR. FITZGERALD: On the other hand, I think 23 1 the witness last indicated, or would, he has 2 expert opinions and would certainly give those 3 to you. So I think it's how you approach the 4 problem. 5 The United States has been repeatedly 6 frustrated in focusing its experts on the case 7 at hand because of the lack of final opinions or 8 conclusions on the part of any of the League's, 9 for example, expert witnesses, most of the 10 Co-op's expert witnesses and I think except 11 perhaps for one expert witness on the part of 12 the Florida Fruit and Vegetable Association, 13 they would not have final opinions either, so -- 14 MR. HYDE: I think -- 15 MR. FITZGERALD: That's a long term 16 problem. 17 MR. HYDE: I think I would disagree with 18 your editorial comments but I just want to make 19 sure we all are operating under the same 20 standard and that our witnesses will be not held 21 to a different standard at such time as they are 22 deposed. That's all I wanted to know. 23 MR. FITZGERALD: You need to take that up 24 with -- if you're unsatisfied or dissatisfied 25 with how DER's experts may respond to 24 1 deposition, you know the remedy. If you're 2 dissatisfied with how the District's might, you 3 know the remedy. If you're ever dissatisfied 4 with how our witnesses designated respond, you 5 can take that up with us. If we're dissatisfied 6 with yours or Miss Ponzoli or any representative 7 of the United States is dissatisfied with the 8 expert witnesses we know the recourse for that. 9 MR. HYDE: I understand Miss Ponzoli to be 10 suggesting what I originally suggested during 11 her deposition of Dr. Russ Rader, (phonetic.) My 12 only concern at this point is not to create 13 problems but to make sure we're all operating 14 under the same understanding in mind. 15 That's enough said on the issue. 16 Would you label that Exhibit 2. 17 (The document was marked 18 Exb. No. 2.) 19 (Whereupon, Mr. Perko entered.) 20 BY MR. HYDE: 21 Q. Mr. Swift, I'd like to show you a document 22 which has been labeled Swift 2. Could you identify 23 that document for me? 24 A. This is a Technical document -- Publication 25 81-5 written in December of 1981 titled, Preliminary 25 1 Investigation of Periphyton and Water Quality 2 Relationships in the Everglades Conservation Areas 3 and it was -- its author is myself. It represents 4 data collected from 1978 through 1979 in Water 5 Conservation Areas 1, 2 and 3, I believe. 6 Q. I'd like you to turn to page 7 of your 7 report where you describe basically the analytical 8 methods that you employed in drafting this paper. 9 Did those methods vary from those used at 10 the time by the District to analyze water samples for 11 routine monitoring? 12 MS. CLEMENTS: Objection. I'm not sure if 13 Dave understands what the routine monitoring was 14 at that time. 15 BY MR. HYDE: 16 Q. Okay. Well, let me back up for just a 17 second and see if I can rephrase it. 18 Do you know what methods were utilized by 19 the Water Management District at the time these 20 investigations were conducted to analyze water 21 samples for routine monitoring? 22 A. In general, yes. 23 Q. Okay. Did the methods that you employed in 24 preparing this paper differ from those analytical 25 methods that were commonly used by the District? 26 1 A. As far as the field methods for collecting 2 water samples, I believe that they were very similar 3 to what our water chemistry lab employed at that 4 time. 5 Q. What about other methods? 6 A. Could you be more specific, other, other -- 7 Q. Are you aware of what is presently used or 8 approved by the Environmental Protection Agency as a 9 standard method for analyzing water quality samples? 10 A. Currently, no. I do not do field work for 11 the last several years, so I'm not up to speed on 12 what the current methodology is. 13 Q. So you wouldn't know how those methods that 14 are EPA approved methods differ or are similar to 15 what you employed -- 16 A. No. 17 Q. -- at that time? 18 A. No, sir. 19 Q. Do you know whether the values that you 20 assigned for nutrient concentrations on the basis of 21 methodology can be compared with other data in the 22 District's water quality data base? 23 A. All of the samples are run through our 24 water chemistry laboratory and the limits of 25 detection were those that were ascribed to all other 27 1 water quality sampling throughout the District. In 2 other words, we simply collected the bottles, 3 prepared them as the Chemistry Department required 4 and we would send these samples in to the laboratory 5 and they would report the results. 6 Q. Can you give me a general description of 7 how that sampling was conducted at that time 8 beginning with your field work? 9 A. Actual collecting samples in the field? 10 Q. Yes. How did you do it? What kind of 11 utensils were used? Things along those lines. 12 A. Yes. We would -- we had a number of 13 stations that were located along a transect. We 14 visited those by airboat. When we moved to a station 15 it was very important that the airboat stop at a good 16 deal distance away from the water quality sampling 17 site because the -- there's a flocculant material, 18 organic detritus, that sort of thing that's on the 19 bottom and it's very easy, for example, for an 20 airboat wave to kick that up. So we would have to 21 stop a good deal distance from the site and basically 22 walk in. We would have to -- we had a plastic 23 sampling bucket that we rinsed three times with 24 de-ionized water and would very carefully take a 25 sample and bring it back to the boat and that -- the 28 1 sample then is filtered and processed according to 2 the laboratory standards right there on site. Then 3 the bottle was put into a laboratory -- or excuse me -- 4 into an ice chest in the dark and they are brought 5 back to the laboratory to be processed within the 6 next day or so. 7 Q. Okay. Did you choose the transects that 8 were utilized for that study? 9 A. Yes, sir. 10 Q. How did you go about choosing those 11 transects? 12 A. We were looking at trying to get -- well, 13 if I can step back a little bit, when I first came to 14 the District I was taken out into Water Conservation 15 Area 2 by Walt Dineen. One of the, I guess, a 16 concern that he had is that the entire Water 17 Conservation Area 2 had a very thick algal mat there. 18 It was thicker than other areas across the Water 19 Conservation Areas. They were kind of concerned what 20 causes that. That was the primary reason I was 21 actually brought in. We did a number of collections 22 of algae samples and water chemistry samples and we 23 basically stumbled upon the problems of difference in 24 algal communities south of the S-10 structures and in 25 the interior. And to figure out why there are not 29 1 algal mats in that area and why there are other algal 2 mats in other areas we decided to take a look at 3 water quality along those transects and take a look 4 at the algal communities along there to see if 5 there's a relationship between the water quality 6 aspect and algal species composition. Therefore we 7 were looking for a gradient downstream of the S-10 8 structures to set up sampling sites and that's 9 basically how we came about it. 10 Q. Were these transect sites a uniform 11 distance apart or were there other factors that were 12 taken into consideration in establishing a given 13 site? 14 A. No. They are generally uniform, but not 15 exactly. It's a very difficult environment to work 16 in, especially back then we didn't have a navigation 17 set, sighting to figure out where we were, nor loran, 18 so, basically, we did the best we could with using 19 aerial maps that we had. It was important, we felt, 20 to have representative sites, though. Just to have 21 one site in a so called nutrient enriched area, we 22 felt we needed several sites. We also needed several 23 sites in background areas and then sites in between. 24 And that's -- from the water quality results that we 25 got we basically said, well, these areas have higher 30 1 nutrients than other areas and that's why we ended up 2 putting a number of sites in a nutrient enriched area 3 and sites in what I called a transitional area and 4 then establish a number of background sites. Area 2 5 is not the only place we looked at. We also did the 6 same thing in Area 1 and we also did the same thing -- 7 similar things in Area 3. 8 Q. When you say Area 1, 2 and 3 are you 9 referring to the Water Conservation Areas? 10 A. Yes. 11 Q. Okay. Perhaps I'm a little bit confused, 12 but I think you said that you wanted to obtain 13 representative sites and you determined what were 14 nutrient enriched sites versus unenriched sites and I 15 presume that you did that by virtue of some degree of 16 water quality sampling. So is it fair to say that 17 you did some sampling to determine what the 18 characteristics of the water were and then came back 19 and chose those sites at a later date? 20 A. In general, yes. 21 Q. Okay. I believe you said earlier that you 22 filtered the samples. Why did you filter them? 23 A. We were looking for total phosphorus which 24 came out unfiltered in the bottle. We were looking 25 at total dissolved phosphorus. That would be -- we 31 1 would filter that in the field. That would be total 2 dissolved phosphorus and we would also get 3 ortho-phosphorus out of another bottle. There are 4 actually three forms of phosphorus that we were 5 sampling from. 6 Q. What kind of filter did you employ? 7 A. A Millipore filter. 8 Q. Millipore? 9 A. Uh huh. It's stated on page 7. 10 Q. Describe the method for testing for 11 dissolved phosphorus or for total phosphorus or for 12 both. 13 A. For both -- 14 Q. When you venture to a given site on your 15 airboat, approximately how far away did you position 16 your airboat from the actual testing site? 17 A. Well, it would vary because some sites were 18 in sloughs. Some sites were in thick sawgrass. Some 19 sites were in very thick cattails. You could get a 20 little bit closer in areas where the vegetation was 21 very thick, but the rule of thumb was to have no 22 disturbance, no wave or anything close to the site 23 and that was paramount because you didn't want to 24 kick up when sampling, you know, particulate matter 25 that you didn't want in your sample so to speak. So 32 1 it could be -- for example, in the 217 gauge, which 2 is a large open slough, that could be 50 feet, for 3 example, from the site and we would taxi up very 4 carefully to the edge of the slough, not get in the 5 slough, because a wave would go across the entire 6 slough. The whole area is very fragile as far as, 7 you know, kicking up material from the bottom. In 8 the nutrient enriched sites and in some thick 9 sawgrass sites you could get a little bit closer, but 10 that was the -- you're really trying very hard not to 11 have any kind of influence being there. 12 Q. You said once you had obtained the samples 13 and filtered them you placed them in an ice chest. 14 A. Uh huh. 15 Q. Was that an ice chest that was cooled or 16 was it just -- 17 A. Yes. 18 Q. -- or just an empty chest? 19 A. Yes. On ice. 20 Q. All right. 21 A. This was the standard practice throughout 22 the District at that time as to -- instead of using 23 mercuric chloride or other toxic substances and I 24 think for safety reasons because they process so many 25 samples, thousands of samples through the laboratory, 33 1 that they felt that kind of preservation was 2 something that they would rather deal with then 3 having to deal with toxic substances for 4 preservation. 5 Q. How was the -- how were those samples kept 6 cold? Was there ice in the chest? 7 A. Yes. Yes. 8 Q. Okay. I believe you stated that you got 9 those samples to your testing laboratories within one 10 to two days. Was there some purpose behind getting 11 them there that quickly? 12 A. Yes. Obviously nitrogen and phosphorus 13 values, if there were particulate matter in the 14 sample, the time -- you know, for example, like 15 bacteria or anything like that, you would want to 16 sample it as quickly as you could, have -- you 17 wouldn't want them sitting around for a week or two 18 and you certainly would want them to be processed as 19 quickly as you could. I believe our sampling usually 20 took place on Tuesdays, that way most of the samples 21 usually got processed by Thursday or Friday so you 22 are looking for -- we bring them back in the ice 23 chest, place them in the District's laboratory. They 24 have a huge cooler that we would put those in. They 25 would be stored, therefore, just before analysis. 34 1 Q. Generally speaking, those samples were 2 actually analyzed by the testing laboratory within 3 two days? 4 A. Uh huh. 5 Q. What do you regard as being the outer 6 limits of storing samples of that nature? 7 A. We did do some -- we did look at that 8 because that was a concern back at that time and, to 9 be honest with you, I can't recall the exact results. 10 I wish I could give it. There was a memoranda that 11 was done. There was a concern about pumping, for 12 example, not in our spot, but some other areas where 13 there was a backpumping study or so forth, so on, but 14 you'd have a lot of particulate matter in the sample 15 and I believe somebody did take a look at shelf life, 16 how long could you hold those. I'm sorry. I don't 17 know the name of the study, but the laboratory did 18 look at it. But, in general, we were trying to 19 process samples within seven days. 20 Q. Could you tell me where in your paper the 21 methodology for testing for total phosphorus is 22 described? 23 A. I don't know if the specifics are in this 24 paper. I believe it goes back to, if you take a look 25 at Water Chemistry Division, what they had in place 35 1 at that time, the standard methods that they were 2 using in the laboratory at that time. I don't 3 believe -- I think it's very generally referred to in 4 this paper. 5 Q. But you're sure that you, in fact, utilized 6 that methodology when testing for total phosphorus? 7 A. Yes, sir. 8 Q. I'd like you to turn to page 18 of Exhibit 9 2. This is the area where you are engaging in a 10 statistical characterization of the data. My first 11 question is what was the limit of detection for your 12 method for testing for phosphorus? 13 A. The -- when we first started, I believe in 14 1978 through 1979 or 1980, it was -- .002 was the 15 limit of detection. I believe in 1980 the laboratory 16 went to .004 is what they ended up doing at that 17 time. So they changed it slightly and I think it was 18 for purposes of they had a very large amount of 19 samples coming in and, for some reason, that 20 particular limit of detection was more appropriate. 21 Maybe they felt that was really as low as they could 22 really go under using their Technicon methodology. 23 Q. When you say .002 and .004 could you relate 24 those two terms to parts per billion? 25 A. Milligrams per liter. 36 1 Q. Can you relate them to parts per billion is 2 my question? 3 A. You mean -- 4 Q. What is .002 milligrams per liter as 5 reflected in a parts per billion gauge? 6 A. I'm talking about two parts per billion. 7 Q. Okay. I want to make sure we're on the 8 same wave length. 9 What methodologies were employed by the 10 District to test for very, very low concentrations of 11 phosphorus -- 12 A. I'm sorry. 13 Q. -- at that time? 14 A. I didn't understand the question. Would 15 you -- 16 Q. What was the methodology employed by the 17 District to test for very, very low nutrient 18 concentrations at the time of this study? 19 A. I'm sorry. I'm a little fuzzy whether 20 Technicon had been incorporated at that time. 21 Q. What else did they use? 22 A. I'd have to go back. I don't recall what 23 the methodology was back at that time. 24 Q. Would one have different concerns in 25 testing for ultra low concentrations of phosphorus as 37 1 opposed to concentrations in the range of 30 to 50 2 parts per billion? 3 A. Um, I think I should probably get this 4 straight is that my area of expertise is not on the 5 water quality monitoring sampling that the District 6 has employed over the years. 7 We utilize it as, you know, a certified 8 laboratory that we utilize, you know, in processing 9 our samples. The District looks very carefully at 10 the quality assurance of the -- you know, achieving 11 what you're talking about; does the number really 12 mean what it says? And they do that, you know, 13 weekly and they do that by looking at very low levels 14 and the water quality lab was satisfied that those 15 levels, you know, were accurate. 16 I can't really tell you too much really 17 specifically about the methodology back at that time. 18 I would refer you to Morris Rosen who worked in the 19 laboratory at that time who could help you quite a 20 bit more with the water quality monitoring program 21 and quality assurance that was employed at that time. 22 Q. So you would defer to a person such as 23 Morris Rosen and others for the District's QA and QC 24 procedures in this regard? 25 A. During that period of time, yes. 38 1 Q. So you wouldn't claim an expertise in the 2 water quality monitoring -- 3 A. I wouldn't claim an expertise in that and 4 Morris is the -- actually the last person that worked 5 in that lab that's still -- you know, is at the 6 District. 7 Q. Okay. How did you use the values below the 8 limits of detection in your statistical analysis? 9 A. We ended up -- converted them up to .004 10 because it just is a very difficult thing to work 11 with because you could get a correlation if you had -- 12 for example, if you had a site that was .002 for two 13 years and then suddenly you had .004 you might get a 14 positive correlation, so what we did is went back 15 through the data and brought it all back up to a 16 .004. In this particular study, I believe we were 17 still relying on the .002 milligram per liter limit 18 of detection. Later what I'm talking about is the 19 publication we put together in 1987. 20 Q. Did your limits of detection change over 21 the years or did the methodologies by which those 22 limits of detection, were they changed? 23 A. As I just answered before, yes, the limits 24 of detection was changed over a period of time. It 25 was either 1980 or '81 it went from, you know .002 39 1 milligrams per liter to .004 milligrams per liter as 2 the limit of total phosphorus and ortho-phosphorus 3 and total dissolved phosphorus. 4 Q. Did your methodologies change in making 5 those determinations? 6 A. Again, you would have to talk to Morris. I 7 am not real sure if Technicon was in operation in 8 1978 or '79. I really can't remember. 9 Q. I'd like you to refer now to page 52. 10 Could you explain to me what a stepwise regression 11 is? 12 A. It's basically looking for relationships -- 13 interlineal relationships, what are the most 14 strongest values. What comes out of that 15 relationship is just a screening tool to look at what 16 are the strongest correlations that could come out of 17 a data set. 18 Q. Is that stepwise regression what you used 19 in presenting the results of your analysis here -- 20 A. On page 52? 21 Q. In your report. 22 A. Well, there are a number of different 23 things that we used. 24 Q. Okay. Did you utilize the stepwise 25 regression? 40 1 A. One of -- that's one of the data screening 2 things we took a look at, yes. 3 Q. Is a stepwise regression sensitive to the 4 order of variable entry into the analysis? 5 A. I'm not all that familiar with that; the 6 specifics of it. I do not know. 7 Q. If you're not familiar with the specifics 8 of the order of variable entry how can you be sure 9 you're employing the stepwise regression properly? 10 A. I can't answer that. 11 Q. Well, could the order of variable entry 12 have influenced the output of your model? 13 A. I have a little problem with variable 14 entry. Could you define that for me? 15 Q. Do you know what variable entry means? If 16 you don't, just tell me. 17 A. I'm trying to ask you what it is, sir. 18 Q. I'm the one that asks questions. You are 19 the one that answers them. 20 A. I don't understand your question, I guess. 21 Q. Do you know what's meant by the phrase, 22 "non-transformed not normally distributed data"? 23 A. Well, the non-transformed data would be -- 24 in our particular case, would be just simply the raw 25 data. We transform data because -- a number of times 41 1 because it was in percentages and is not normally 2 distributed. 3 Q. Well, how does the use of non-transformed 4 not normally distributed data influence 5 interpretations of the data in your analysis as 6 compared to later analysis of that same data? 7 A. A lot of biological data is not normally 8 distributed often. It's skewed to the left and 9 you're working in magnitude differences between 10 samples and it's -- for example, the cell density 11 data that we used, it would be -- if you took a look 12 at it, sometimes it was not automatically 13 distributed; you needed to transfer it to a log to 14 the assumption of trying to look at correlation 15 coefficients, for example. You would like to have 16 your data normally distributed. And the cell, for 17 example, the percent composition of cell data, for 18 example, is normally handled by an arcsine 19 transformation so that you have normally distributed 20 data or at least have attempted to put that 21 information together so it is at least close to a 22 normal distribution. 23 Q. Okay. What did you mean by the phrase, 24 "skewed to the left"? 25 A. For example, a lot of the data may be -- if 42 1 you plotted it, for example, it could be all columned 2 down on the left-hand corner of XY axis, for example. 3 It does not show up a whole range of values that you 4 would be expecting to take a look at. 5 Q. Of all the factors that you measured and 6 evaluated statistically which one or group was the 7 main or determinate of periphyton occurrence? 8 A. The primary thing we took a look at and 9 it's because we looked at all three Water 10 Conservation Areas, major ions, the amount of 11 dissolved minerals, for example, that were in the 12 water were the primary factor that was responsible 13 for differences in species composition between sites. 14 We also found that pH and total phosphorus were also 15 important factors. pH was related primarily to the 16 fact that whether it was an alkaline or -- excuse me -- 17 whether it was hard water site, those were generally 18 alkaline sites. Soft water sites were usually acidic 19 sites so that parameter pretty much related to each 20 other. Phosphorus concentrations were related to the 21 occurrence of certain specialized community of algae 22 that occurred within an area south of the S-10 23 structures. 24 Q. To what do you attribute the presence of 25 major ions? 43 1 A. Major ions, what is interesting about the 2 study is that you could -- all three Water 3 Conservation Areas had widely varying major ion 4 concentrations. 5 In the interior of Water Conservation Area 6 1 major ion concentrations were very low for a number 7 of factors. Number one, the vegetation and the 8 elevation of the marsh, if you could kind of think of 9 the marsh sort of as a dome, so to speak, water 10 rainfall would fall and basically not be mixed with 11 the surrounding water that was discharged around the 12 periphery of Area 1. The thick peat in Area 1 also 13 prevented ground water from mixing with the surface 14 water so as a result the low ionic content of the 15 water in Area 1 or as a result of looking at the 16 ionic content of the water in Area 1 was also 17 basically from rainfall. 18 Area 2 is a very different scenario and if 19 I could compare Area 2 and Area 3, you will find that 20 chlorides and sodium and calcium were the dominant 21 major ions in Area 2 and then if you looked at Area 3 22 you would find calcium, calcium carbonate were the 23 dominant ions, but sodium chlorides were low. The 24 reason for that is Area 2 receives, has -- at that 25 time it had three direct inflow structures that 44 1 supplied water from the Hillsboro Canal and that 2 drains both the Everglades Agricultural Area which 3 historically contains large amounts of connate 4 seawater. In other words, there's a ancient sea bed 5 there and when you pump water from the ground it 6 contains high sodium chloride levels and those are 7 pumped south and they move through the Hillsboro 8 Canal into Area 2. And I think if you look at a 9 water budget for Area 2, maybe 40 to 50 percent of 10 the inflow water comes from the Hillsboro Canal. So 11 as a result you have a lot of chlorides and sodium 12 that kind of trace its origin, really. So we have 13 Area 1 it's origin is primarily -- the interior is 14 rainfall. And Area 2, a good portion is rainfall 15 plus inflow water and Area 3 is primarily -- at least 16 the open marsh is primarily rainfall. 17 Q. Does the presence of the artificially 18 constructed canals in these Water Conservation Areas 19 have any significance or relationship to the presence 20 of major ions? 21 A. Yes. It's a vehicle for transport. You 22 can actually see sodium and chloride transported all 23 the way from the Everglades Agricultural Area and as 24 a result it shows up in deep areas in Everglades 25 National Park and it's not reported by me, but 45 1 reported by Klein in 1975. 2 Q. Does the depth of the canals have any 3 significance concerning the presence of major ions? 4 A. I do not know. 5 Q. Do you know whether the canals are having 6 any influence or impact on the movement of major ions 7 from the ground water into the surface waters of the 8 Water Conservation Areas? 9 A. I do not know. 10 Q. Let's back up for a moment. Do you know 11 what a factor analysis is? 12 A. Uh huh. 13 Q. Did you employ a factor analysis in 14 preparing this report? 15 A. Um, I don't recall if this was it or not. 16 Oh, yeah. Sorry. Yes. 17 Q. How did or how does a factor analysis give 18 you the information by which you can relate water 19 quality data to periphyton occurrence? 20 A. Well, we're using a factor analysis trying 21 to use it as a screening tool to look at what 22 variables are closely related and in that factor 23 analysis I had responded to earlier what -- what -- 24 what parameters ended up being the most important as 25 far as major ions and that sort of thing. That was 46 1 also a tool that we used to basically screen the data 2 and look for correlations between our parameters. 3 Q. Does the order in which the variables are 4 brought into the statistical analysis affect the 5 outcome of that analysis? 6 A. It could, yes. 7 Q. How could it affect it? 8 A. Well, the basic problem is -- I think we'd 9 have to take a look at auto-correlation -- is that 10 you don't have really similar factors outweighing 11 other factors. In other words, if you had a whole 12 bunch of factors that were related to one another, 13 they would tend to drive it to a conclusion that 14 those were the dominant things going on there and 15 that's something you have to watch out for. 16 Q. What did you mean by the phrase or term, 17 "auto-correlation"? 18 A. I was trying to explain it where you'd have 19 similar parameters that were related very 20 specifically and if they are brought together they 21 can have some affect on the -- your results. 22 Q. I'd like you to turn to page 63 of your 23 report. I quote, "Phosphorus and nitrogen were both 24 potentially growth limiting nutrients at interior WCA 25 sites due to their low availability." 47 1 Do you still agree with that statement? 2 A. Yes. 3 Q. Okay. Does your research, however, support 4 a sole focus on phosphorus as the growth limiting 5 factor in the Everglades? 6 A. I don't believe it does. It looks at 7 nitrogen also. Because we sampled for the nitrogen 8 at the same time. 9 Q. What chemical forms of nitrogen comprise 10 the inorganic nitrogen that you used as a factor in 11 your statistical analysis? 12 A. Let's see. I'm trying to remember. I 13 believe it was ammonia, what we call NOX which is a 14 calculation of nitrate plus nitrite. 15 Q. Is that all? 16 A. I believe so. I believe that's basically 17 what we were looking at. 18 Q. Did those forms of inorganic nitrogen 19 accurately or adequately characterize the forms of 20 nitrogen that are used primarily by periphyton? 21 A. I believe so. 22 Q. Okay. 23 A. We felt they did in that study, yes. 24 Q. What other types of inorganic nitrogen 25 might one measure to determine what is used by 48 1 periphyton? 2 A. I think I answered the question. Maybe I 3 don't understand your question. 4 Q. Let me see if I can rephrase it. 5 Are there any other types of inorganic 6 nitrogen that are utilized by periphyton other than 7 the ones you just listed? 8 A. In a soluble form, as I understand it, 9 those are the major ones that we felt that were 10 important. 11 Q. Okay. Does a variation in the proportional 12 composition of inorganic nitrogen influence the 13 availability of nitrogen to periphyton? 14 A. Are you talking about for example -- I 15 guess I need to understand. Could you give me an 16 example? You want to know if there is a composition 17 that is higher than others? Is that what you are 18 saying? 19 Q. Yes. 20 A. It's possible. Ammonia, for example, may 21 be utilized by some species very quickly, whereas 22 nitrate -- or it depends on the species. I don't 23 pretend to be expert on the uptake efficiencies of 24 nitrogen by algae, so I don't think I could really 25 answer your question as an expert in that particular 49 1 area. 2 Q. So you would disclaim any expertise in that 3 particular regard? 4 A. As far as the uptake efficiencies of algae 5 as far as in regards to nitrogen uptake, yes. 6 Q. Okay. Let's back up for just a moment. 7 What factors were, by your term, auto-correlated? 8 A. Which ones? 9 Q. Yes. 10 A. Well, things like where we had sodium and 11 chlorides, for example. That's really looking at the 12 same thing. 13 Q. What influence do inorganic ions have on 14 periphyton species and communities? 15 A. If you're -- any specific one? Because 16 they vary. Could you give me a specific ion? 17 Q. Okay. Can you relate it to chloride ions? 18 A. Yes. The literature, for example, chloride 19 in saltwater versus freshwater, high concentrations 20 of chlorides do limit some algal species and the -- 21 their cell structures cannot handle some high 22 concentrations of chloride whereas others can and you -- 23 when you look, for example, at the diatoms and, for 24 example, at a type of algae, they are very -- they 25 are distributed along gradients of chloride 50 1 concentrations. Usually you're talking about 2 chloride concentrations going from seawater to 3 brackish water to freshwater. In this particular 4 study chlorides between the sites, for example, may 5 or may not have had an impact. 6 Q. Did you utilize chloride ions to correlate 7 all your ions in your study? 8 A. No. 9 Q. Okay. I believe you said awhile ago that 10 you attributed these chlorides to connate water that 11 underlay the Everglades Agricultural Area. 12 A. Yes. 13 Q. Is it fair to say, then, that the presence 14 of these connate chloride ions is attributable to the 15 construction of the project itself? 16 A. No. All I can tell you is that's the 17 origin of that water of those ions. 18 Q. Well, how else would have they have gotten 19 to the Water Conservation Areas but for the 20 construction of the project? 21 MS. CLEMENTS: Objection. Speculation. 22 BY MR. HYDE: 23 Q. You may answer the question. 24 A. Could you repeat the question, please. 25 Q. How else would have they have gotten to the 51 1 Water Conservation Areas but for the construction of 2 the project? 3 A. You're assuming that the project is the 4 only source. The largest source that I understand is 5 the daily pumping of water from the EAA for, you 6 know, agricultural pumping. That water moving 7 through the fields, moving through the ground water 8 into fields is probably the primary source of where 9 that is coming from. 10 Q. Pumping of what water? 11 A. Irrigation water from canals. 12 Q. Okay. 13 A. Not constructed as part of the project. I 14 think you're trying to say, if I understand your 15 question, is that the primary source would be the 16 Central and Southern Florida canal system and I don't 17 think that's entirely true. 18 Q. Well, is this connate water present in the 19 agricultural canals within the EAA? 20 A. I understand that it is. 21 Q. How did it come to be present there? 22 A. I understand that it was an ancient sea bed 23 bottom that dried up periodically and as a result 24 salts and other deposits formed in the limestone 25 bedrock. 52 1 I would like to say that I am testifying, 2 obviously, out of my area of expertise, but the 3 information I'm repeating comes from Gleason, Patrick 4 Gleason's publications, Klein, 1975; I believe 5 McPherson, some other investigators who have looked 6 at water quality relationships between the EAA and 7 Water Conservation Areas and found similar results. 8 Q. Let me go back to my question. How would 9 these chloride ions come to be in the Water 10 Conservation Areas if it were not for the project 11 itself delivering those waters to the Water 12 Conservation Areas? 13 MR. FITZGERALD: Objection. Asked and 14 answered. Calls for speculation. 15 MS. CLEMENTS: You can go ahead. 16 THE WITNESS: I had asked -- answered that. 17 And it is basically that, as I understand, the 18 system was developed, the Central and Southern 19 Florida project to, one, provide -- open up the 20 Everglades for agricultural development and one 21 of the things that resulted from that is, 22 obviously, the conversation areas were set aside 23 as areas for surface water runoff to be 24 discharged during storm events and, yes, that 25 the water is transported from the EAA field into 53 1 the Water Conservation Areas and that is 2 basically under what's called Bulletin -- I 3 think it's 442 out of the Belle Glade Experiment 4 System. That's how the system was originally 5 devised is dividing up the Water Conversation 6 Areas in the Everglades Agricultural Area, 7 originally looking at what kinds of soil types 8 would be good for agriculture; what types of 9 soil types would not be good for agriculture, 10 but should be set aside for future preservation. 11 BY MR. HYDE: 12 Q. Considering that answer, would it be fair 13 to characterize the project canals and structures as 14 being delivery systems for these waters into the 15 Water Conversation Areas? 16 A. They do move water from the Everglades 17 Agricultural Areas into the Water Conservation Areas, 18 yes. 19 Q. Okay. What, to your mind's light, is more 20 important ecologically speaking, considering the use 21 of nutrients or the issue of nutrients in periphyton, 22 growth rates of periphyton or community structure and 23 species occurrence? 24 A. Well, they are both really related, I 25 think. 54 1 Could you repeat the question? I'm trying 2 to figure out what the separation is that you're 3 trying to get at. 4 Q. What, by your mind's light, is more 5 important in an ecological sense, considering the 6 nutrients and periphyton on the one hand, the growth 7 rates of periphyton on the other hand or the 8 community structure and species occurrence? 9 A. In my view, you really have to look at the 10 whole -- whole -- both issues and maybe some other 11 issues also. Trying to look at -- before you define 12 whether it's good or bad, you need to take a look at 13 the species composition. You need to take a look at 14 biomass production or algal growth rates in this 15 particular since. You need to take a look at 16 productivity and try to understand, you know, how 17 that impacts the entire ecosystem because they all 18 are related to one another and you need to look at 19 all of those factors, really, in total. 20 It's -- it's kind of an unfair question 21 because it's kind of like you asking to spit on the 22 flag or spit on the Bible. You really have to -- 23 Q. That's an interesting metaphor. 24 A. You have to really look at it as a total 25 system and evaluate all of those different 55 1 parameters. 2 Q. Does this particular paper purport to 3 address any of those concerns, growth rates of 4 periphyton, community structure or species 5 occurrence? 6 A. Yes. 7 Q. All of them? 8 A. Looks at growth rates as algae grown in 9 glass slides. It looks at periphyton species 10 composition. 11 What was the third? 12 Q. Species occurrence. 13 A. Species occurrence, yes. 14 Q. Do the tests that you employed in preparing 15 this paper establish that phosphorus and not 16 inorganic ions are the most important substances that 17 control diversity and abundance of periphyton species 18 in the WCAs? 19 A. No. The paper identified major ions as the 20 major factor based on the analysis that we had done 21 here and the reason for that is that there's such a 22 wide variation in major ion content between the Water 23 Conservation Areas that that became the dominant 24 factor that we were looking at. I would say that, 25 for example, if we had not included Water 56 1 Conservation Area 1 interior information, that we 2 probably would have both major ions and pH drop back 3 as major factors and nutrient content would be the 4 primary driving factor in determining species 5 composition. But this paper clearly demonstrates 6 that the primary factor, looking at all of the Water 7 Conservation Areas was major ion content. 8 Q. I'm struggling a little bit with asking the 9 next question, but do you believe there to be, quote, 10 good types of blue-green algae and bad types of 11 blue-green algae in the Water Conservation Areas? 12 A. You are struggling with that question. 13 In reflection to being bad for the 14 environment -- or excuse me -- bad being bad for the 15 environment, good being good for the environment, is 16 that what you're trying to get at? 17 Q. Yes. I think you could characterize it as 18 good versus bad to the environment or as being 19 reflective of natural conditions versus artificially 20 induced conditions. 21 A. If I could, I would turn the question 22 around a little bit and answer it this way. The -- 23 what we were trying to do is identify is there a 24 relationship between the species composition of 25 algae, their growth rates and water quality factors? 57 1 That was the purpose of the study and we were simply 2 reporting these algae occur in this kind of water 3 quality. These algae occur in another. As far as 4 good or bad, I don't know if you would put it in that 5 context, but what you would put is these reflect this 6 kind of environment, whether it's a low nutrient 7 alkaline hard water environment or whether it's a 8 high nutrient alkaline hard water environment or 9 whether it's a low nutrient low ionic content 10 environment and that's what we were trying to achieve 11 there. 12 Q. Do you have any opinion as to whether there 13 is a difference in nutritional value between these 14 different types of blue-green algae? 15 A. No. No one's doing anything on that yet. 16 Q. Do you know if anyone is studying that? 17 A. I was under the impression that Bill Loftus 18 and Everglades National Park had taken some sort of 19 look at the stomach contents of Everglades flora -- 20 or excuse me -- fauna, whether it be tadpoles or 21 whatever. Joan Browder had done a little bit of work 22 on tadpoles, I think, but I know of no real specific 23 study that really gets at whether the -- you know, 24 blue-greens are better than diatoms or whatever in 25 the Everglades. 58 1 Q. And do you claim any expertise in that 2 regard, then? 3 A. No. 4 Q. Do you consider the interior marsh areas, 5 "closed" systems? 6 A. Well, closed in that rainfall is the 7 primary nutrient inflow. I think there's a lot of -- 8 we call it the River of Grass and it's no longer a 9 river. There are compartments of grass, I guess. 10 There are in -- Area 2, for example, is a different 11 situation because we do have a lot of flow through 12 that particular system that comes from an external 13 source. Area 1 is a little different than Area 2 14 because it has a flow component around its peripheral 15 areas and the interior is primarily rainfall driven; 16 closed in this particular manner. I meant closed 17 with respect to nutrient inputs. I felt rainfall -- 18 at least the large Water Conversation Area, Area 3, 19 for example, was primarily rainfall driven. 20 Q. Is that the only factor that you're 21 utilizing this term "closed" to mean? 22 A. Yes. Yes. 23 Q. Okay. Is there a geographical component to 24 a system or any of these systems being "closed"? 25 A. Geographically, some areas of the 59 1 Everglades are more compartmentalized than others. 2 The -- I think I just was saying that in Area 3 there 3 are some small components that are totally enclosed 4 by berms. They are small areas, but in general it's 5 a hard thing to describe because Area 3, for example, 6 has the Miami Canal running down the middle of it. 7 It probably does not contribute that much water as 8 far as surface water over the entire marsh. Rainfall 9 is the primary inflow factor there. Again, that's 10 what I was trying to get at. 11 Q. Let me back up just for a second. 12 Concerning the time period between collection and 13 analytical analyses for orthophosphate, nitrate and 14 total dissolved phosphorus, were they all done in the 15 same time periods that you alluded to earlier? 16 A. What did I allude to earlier? 17 Q. Approximately two days -- 18 A. Yes. 19 Q. -- maximum to a week? 20 A. Yes. 21 Q. All right. Were surface water samples 22 filtered through Millipore membrane filters? 23 A. I believe so. On page 7 I think it states 24 that. 25 Q. I'd like you to refer to Table C-6. I'm 60 1 not sure which page that's on. C-7. Excuse me. 2 It's on page C-7. 3 What role, if any, did water depth play in 4 your stepwise multiple regression? 5 A. I would have to look at it a little bit. I 6 haven't seen this in quite a few years. 7 Q. At the bottom of the page, and I guess it's 8 like foot note (a), it identifies water depth as 9 being one of the independent predictor variables 10 included. 11 A. In the table I don't see it as a -- unless 12 you see it I don't see it as a big factor. 13 Q. Okay. What about hydroperiod length? 14 A. Well, it must be remembered what we were 15 doing is looking at algal growth on a glass slide 16 incubated for a specific -- actually a relative short 17 period of time, approximately 45 to 60 days -- I 18 believe 60 days -- and really -- and we grew these -- 19 grew the algae on the glass slides, which would have 20 the same amount of light, were at the same depth 21 within the water column and basically all of those 22 parameters were all the same. What we were hoping to 23 look at is differences in water quality at each site; 24 trying to rule those things out. That was the 25 purpose of using floating glass slides so water depth 61 1 and that sort of thing was similar. We did throw in, 2 however, hydroperiod length for the number of days 3 dry, the number of days wet. The water depth we 4 actually recorded at the sites. We threw that into 5 the particular equation, into this coefficient. It 6 didn't have much to do with what we came out with. 7 Q. Do you recall whether there was a 8 correlation coefficient for hydroperiod length and 9 water depth? 10 A. No. 11 You mean did we look at it? 12 Q. Yes. 13 A. We did look at it but nothing came out of 14 it. 15 Q. So you didn't establish one? 16 A. No. We did not find a significant 17 correlation that existed between them. 18 Q. Okay. How many stations did you sample at 19 in conducting this study? 20 A. This one? 21 Q. Yes. 22 A. I'm guessing 18. I can't remember. 23 Q. How many samples did you take per station? 24 A. Let's see. Over the incubation period from -- 25 I believe from four to five during the incubation 62 1 period. Talking about water quality samples. Then 2 as far as the chlorophyll a samples I believe we 3 looked at five, measured five slides for chlorophyll 4 a and a minimum of two slides at every station for 5 species composition. We had devised a thing called 6 an ALGAESTAT program which was a physical way to look 7 at the data and we would have a preserve slide that -- 8 or preserve sample of algae we would look at and look 9 at the percent co-variation and if it was greater 10 than that we would do another count. This is for a 11 dominant species so we knew we would have a 12 representative species composition information. 13 Q. Okay. How many times did this sampling 14 occur? 15 A. Let's see. This was primarily during the 16 summer of 1978-'79, so that you're talking about a 17 summer. I don't remember how many weeks that was. 18 Q. What were the environmental conditions 19 present at that time? 20 A. In 1978 and '79 it was a relatively deep 21 water system and Area 2 was much deeper in 1980, '81, 22 '82, '83, '84. 23 Q. How did that change or how would that 24 change in water depth affect your analysis if at all? 25 A. Again, as I was trying to relate, we were 63 1 looking at various short term colonization of algae 2 on slides and the hydroperiod factor would not come 3 into play simply because we were only looking at 4 collecting algae on glass slides over a 60 day 5 period. So hydroperiod, if it was wet, obviously 6 there would not be a -- hydroperiod would not be an 7 issue between the sites. And I'm not sure if I 8 answered your question. I kind of wandered. 9 Q. I think you did. 10 Was nitrogen enrichment of your filtered 11 samples detected? 12 A. Nitrogen enrichment? Um, in the -- one 13 interesting thing we found from the water quality 14 information we were looking at is nitrate would drop 15 to very, very low levels very quickly in the marsh 16 and it's probably to do with -- we're just guessing -- 17 there's a lot of bacteria, other kinds of nitrifying 18 and de-nitrifying processes going on in the marsh at 19 that time and nitrate levels were relatively low as 20 compared to the canals; very frequently at detection 21 levels. 22 Q. Okay. Did you measure water depth at your 23 sample sites? 24 A. Yes. 25 Q. How did you determine water depth? 64 1 A. We very simply looked at some sticks that 2 we had placed in the ground and we knew, relating 3 them back to gauges, roughly what the water depths 4 were. We also, you know, would take samples with -- 5 made a ruler with a very large piece of a board with 6 plywood on the bottom just to get relative depths 7 also across the slough that we were looking at too. 8 Q. Did you measure the depth of the peat at 9 these sampling sites? 10 A. I believe we did. It's somewhere. I don't 11 know where it is. 12 Q. How does one go about determining peat 13 depth? 14 A. We had a long rod that we would simply 15 stick down into the peat, subtract away the depth of 16 the water. 17 Q. Do you just manually shove it into the 18 peat? 19 A. Yes. At a number of different places. It 20 was just a general -- we were just generally looking 21 at it, what the differences between the sites were. 22 Q. Did your sample stations differ in the 23 species composition of the plants that were there? 24 A. Yes. 25 Q. Were they representative of different 65 1 species compositions in the sense of having one being 2 a macrophyte or sawgrass dominant sighted site, 3 another being a cattail dominant sighted site, the 4 third being maybe a plant species characteristic of 5 an open slough? 6 A. Yes. 7 Q. Are all of those sampling sites described 8 in this report as being one or the other of these 9 categories? 10 A. I think so. I believe each site is 11 described if I'm not mistaken. I might be mistaken. 12 Page A-2 through A-4. 13 Q. Table A-1, which is on page A-2 for some 14 curious reason, indicates or suggests that some sites 15 were sampled only once, is that correct? 16 A. Yes. 17 Q. How did you use this type of data in your 18 water quality analyses and species composition 19 analyses? 20 A. I think we did not use them in some cases. 21 I think like, for example, B-1, for example, in Area 22 2, it was just such a rank and difficult spot to get 23 to at low water. There were very thick mats of 24 vegetation that were -- well, they were impossible to 25 get through with an airboat. We got stuck a bunch of 66 1 times. We felt we should not go back and sample 2 those just from safety sake. We had to chop our way 3 out of it several times. 4 Basically what I tried to do in this table 5 is identify every place we looked at and -- but we 6 did not include necessarily every station in our 7 analysis. 8 Q. Okay. I'd like you to turn to page 72 and 9 take you through. 10 Number 2 in your summary on that page 11 reflects, and I quote, "Earlier periphyton studies in 12 the Everglades have shown that these microorganisms 13 are an important component of the marsh food chain." 14 Which studies are you referring to here if 15 you can identify them? 16 A. Number 42 on page 78, Kolopinski and Higer. 17 I just want to look at some others, if I can just go 18 through here. 19 Q. Take your time. 20 By the way, if you wish to take a break at 21 any time, please feel free to request one. 22 A. I think number 27, Gleason and Spackman. I 23 believe they mentioned it. I think number 32, Hunt 24 1961. Sorry I'm taking so long. I'm not familiar 25 with this any more. Number 99, Van Meter, 1965; 100, 67 1 Van Meter-Kasanof and Wood and Maynard, 1974, number 2 114. 3 Q. Are you of the opinion that periphyton is 4 an important component of the marsh food chain? 5 A. Yes. 6 Q. What's the basis for that opinion? 7 A. Well, there's probably only three types of 8 how you pass energy up the food chain and that would 9 be you would have to have plankton which are floating 10 microorganisms in the water column. Those are not 11 present in abundance in the Everglades. 12 The second area would be the algal 13 community such as periphyton. 14 And the third would be the detrital 15 components of sawgrass and of other products and 16 because this is such a conspicuous feature, diatoms, 17 for example, and a number of green algae are present, 18 these are in every other ecosystem -- aquatic 19 ecosystem, these are grazed upon by invertebrates and 20 fishes and that sort of thing. 21 So this is a very similar case. 22 Periphyton, for example, in the literal zones of 23 lakes world wide are felt to be an important 24 component of those aquatic systems. 25 So not knowing whether that detrital 68 1 component of sawgrass or other marsh vegetation, what 2 role, how important each one is, no one has 3 determined that as yet. I would believe that 4 periphyton would play a very important role. 5 Q. Does it make any difference what kind of 6 periphyton is in this marsh food chain as long as 7 it's present there? 8 A. I would think that it might. For example, 9 in other communities, blue-green algae are utilized 10 and passed directly through the gut and they may not 11 necessarily be -- you know, get an energy requirement 12 from it, but that is recycled back in the food chain. 13 In the Everglades diatoms and green algae 14 and other types of organisms that are found in other 15 environments that are nutritional algae are present 16 everywhere and in some degree of the productivity of 17 that system I would assume it would depend on that. 18 No one has done a quantitative study, to my 19 knowledge, at this point, of the exact quantitative 20 role that periphyton do play. 21 Q. Do you know whether anybody's conducting 22 such a study? 23 A. I don't know. 24 Q. Is that the reason why your answer was a 25 little bit qualified by your use of word "may" as 69 1 opposed to being firm? 2 A. I believe I said, "may" in several cases in 3 here. 4 Q. Some people use "may" to mean different 5 things. 6 A. I understand. 7 Q. Do you regard the Everglades ecosystem as 8 being a detrital food base system? 9 A. Well, if you include the periphyton 10 component as part of that detrital food base, yes. 11 Q. What do you mean by detrital food base? 12 A. Well, the -- for example, the -- in that 13 particular sense, it would be the components that 14 would be derived by the periphyton algae that may be 15 consumed and in the Everglades there is a calcium 16 carbonate, actually, calcium carbonate -- strike 17 that. There is an algal mat that is made up of algae 18 and Utricularia which are bladderwort and forms a 19 very large mat in that system and components of that 20 system could be grazed upon and that's what I'm 21 talking about is the detrital component in the 22 Everglades. 23 Q. The next observation here number 3 reads, 24 "In addition to their importance as a food source, 25 periphyton photosynthesis and metabolism greatly 70 1 influence marsh water diurnal dissolved oxygen 2 concentrations and calcium carbonate (marl) 3 deposition in marsh sediments." 4 First could you explain to me how 5 periphyton photosynthesis and metabolism influences 6 diurnal water or dissolved oxygen concentrations? 7 A. In the presence of sunlight the periphyton 8 photosynthesize the uptake carbon dioxide from the 9 water column and as a by-product oxygen is produced 10 during the day and you can see this in dissolved 11 oxygen studies out of the marsh which I have done and 12 you can see in open water sloughs very large mats, 13 you see a very large swing in diurnal oxygen 14 concentrations in the marsh and what I believe -- I 15 had worked with Tom Blancher at FIT doing a contract 16 for us -- and what I believe he found is that 17 atmospheric re-aeration of the water and periphyton 18 and photosynthesis were the primary sources of oxygen 19 production in the marsh. 20 Q. Okay. 21 A. If the calcium carbonate -- I can explain 22 as calcium or as carbon dioxide -- is taken out of 23 the water column that shifts the carbonate system to 24 the right and having as a result the calcium 25 carbonate is precipitated on the outside cell sheets 71 1 of the algae of the Everglades and that's where marl 2 production is formed in the system and as a result of 3 that we have marl soils that predominate in various 4 areas of the Everglades. 5 Q. Does calcium carbonate affect the use of 6 periphyton as a food source? 7 A. I do not know. 8 Q. What did you mean in your last answer or 9 the answer before last by "shift to the right"? 10 A. Um, in an equation of the carbonate 11 equation, as a result of carbon dioxide uptake in the 12 system, you will have a calcium carbonate precipitate 13 as a result of that and as a result of the photo -- 14 algal photosynthesis. 15 Q. Okay. The next summary point, number 4, 16 reflects, "Recent nitrogen fixation studies in the 17 Water Conversation Areas show that periphyton may 18 play an important role in the marsh nitrogen cycle." 19 By use of the word "may" again, that's a 20 rather tentative conclusion. Is it still tentative 21 in your mind? 22 A. The information of where it comes from is a 23 study done by Antioch College by a fellow by the name 24 of Jeff Goldberg, I think, and they had done some 25 nitro fixation experiments of the mat in the Water 72 1 Conversation Areas. 2 The other observation I made is that the -- 3 there's a algae that makes up the mat out there. 4 It's called Scytonema hofmannii and it contains 5 heterolysis. Heterolysis are often thought sites of 6 nitrogen fixation. What role, as far as a nitrogen 7 source in this system, it's not really clear. I just 8 could tell you that in India, for example, the same 9 species or very similar species are used to -- when 10 they flood a rice field, for example, they'll build 11 up this big algal mat and then they'll drain the 12 marsh, harvest the rice and then plow it under and 13 that mat ends up being a nitrogen source for those 14 systems. The Everglades could play a similar role. 15 Q. Do you have any opinions within a 16 reasonable degree of scientific certainty at this 17 time as to whether periphyton does play an important 18 role in the Everglades marsh nitrogen cycle? 19 A. No. 20 Q. Statement 6 reads, "Although periphyton 21 algae are recognized as an important component of the 22 Water Conversation Area ecosystem, little is known 23 concerning their seasonal population dynamics, 24 species composition, growth rates or their 25 relationship with water quality parameters." 73 1 Do you continue to agree with that 2 statement? 3 A. We know more and that was the purpose of 4 the study is to take a look at species composition, 5 growth rates and how they related to water quality 6 parameters. 7 Q. Okay. This study, that is Exhibit 2, was a 8 summer study, was it not? 9 A. Yes. 10 Q. Would you expect seasonal differences as a 11 result of this study? 12 A. Yeah. I think number 6 was actually trying 13 to convince my boss that we ought to do -- take a 14 look at seasonal and we did. That was what we did 15 next, actually, looked at seasonal aspects of it. 16 Q. Would seasonal differences affect the 17 statistical outcome of your analysis? 18 A. From the standpoint -- I think it would. I 19 think you would have, for example, differences in 20 biomass during low light, low temperature periods. 21 You would have less biomass than during -- that being 22 during the winter than you would have during the 23 summer. 24 Q. I think you just said, "That's what we did 25 next." What were you referring to there? 74 1 A. We continued sampling from 1980 to 1982 and 2 we looked at -- attempted to look at monthly growth 3 rates on slides and what we found there was that the 4 end of the wet season was the period of greatest 5 production in the system. I think it's related back 6 to the interior sites, greater rainfall, possibly 7 more nutrients in the system. Also high light was 8 also a factor. 9 Q. Was this later sampling a year round 10 sampling effort? 11 A. Yes. 12 Q. Okay. I'd like you to turn to the next 13 page, number 12. The second sentence in that 14 observation reads, "Although water depth and 15 hydroperiod length were poorly correlated with the 16 development of periphyton communities on glass slides 17 during 1978-'79, it is well documented that extended 18 hydroperiod length encouraged the long term 19 accumulation of periphyton biomass (standing crop) in 20 some portions of the Everglades." 21 What is the basis or what is the 22 documentation for that observation? 23 A. I believe it's Maynard and Wood that I 24 quoted before. It's in here. 25 Q. This is in your list of references? 75 1 A. The name is Woodward or Maynard. Excuse 2 me. Wood and Maynard, 1974, reference number 114. 3 Q. What significance, if any, does this long 4 term accumulation of periphyton biomass have? 5 A. Well, the point of the statement was -- is 6 that there is a relationship between hydroperiod and 7 periphyton biomass and our study was not looking 8 directly at the effects of hydroperiod. It was 9 trying to focus on the effects of water quality. 10 Q. Might that documentation have also included 11 Van Meter's work? 12 A. It may have. I remember the specific 13 statement about the size of mats and that sort of 14 thing and about the size of the mats in the Wood and 15 Maynard paper. Van meter may have also said 16 something about that. 17 Q. I'd like you to turn to page 19, Table 1. 18 A. Okay. 19 Q. About half way down the page you've listed 20 total phosphorus listed as Total P, I presume. 21 A. Uh huh. 22 Q. But it doesn't appear that you analyzed 23 total phosphorus, is that correct? 24 A. No. We analyzed total phosphorus, I 25 believe. 76 1 Q. What were the ranges and sample size? 2 A. I don't recall. 3 Q. Would you regard the data as being normally 4 distributed? 5 A. As far as total phosphorus is concerned? 6 Q. Yes. 7 A. The data being total -- being total 8 phosphorus data, as I recall, the best of my 9 knowledge, if you look at the actual data that was 10 collected, I think we started out trying to figure 11 out if -- which phosphorus parameter was the one that 12 would be most useful to us and I believe total 13 dissolved phosphorus ended up being that because it 14 did not seem to fluctuate all over the place and we 15 felt, I think, that the total phosphorus values might 16 have been impacted by some sort of, you know, either 17 particulates or whatever that were in the water 18 column at the time. I believe if you look at the 19 data base, I think -- I'm not clear -- I think the 20 first several sets of samples we did not look at 21 total phosphorus directly and that we looked at total 22 dissolved phosphorus, but I think later we looked at 23 all of them. 24 Q. Okay. I'd like you to turn now to page 29 25 concerning discussion of a cluster analysis of the 77 1 periphyton cell volume data. 2 A. Uh huh. 3 Q. Was this analysis only based on this summer 4 data? 5 A. Yes. 6 Q. Okay. Does it mean only one sample time or 7 did it reflect only one sample time? 8 A. No. I think it was both -- well, I'm not 9 real sure. Let me look. 10 Is there a table where the results are 11 presented? 12 Q. It reflects Appendix B. It's on page B-1. 13 A. Yes. It says in B-2, "Figure B-1 presents 14 the results of the cluster analysis of the summer 15 1978 periphyton data." I thought it was both 16 periods. 17 Q. On how many sites was this cluster analysis 18 conducted? 19 A. Take a look at B-3. You can count them. 20 18. 21 Q. Okay. Was this just one sample per year? 22 A. I apologize again. I haven't looked at 23 this for quite awhile. You're question was: Was 24 this based on one sample per year? 25 Q. Yes. 78 1 A. Does that mean -- I think what it was based 2 on is the results of the summer 1978 data. In other 3 words, the results of the summer efforts. 4 Q. Okay. Well, how many times did you site 5 the periphyton samples and pick them up again? 6 A. Well, again, it should be in the methods 7 section. 8 A. Well, I can't really be accurate. I've 9 forgotten exactly what we did in 1978, whether we put 10 them out in June or July and picked them up in 11 August. What would reflect that, however, would be 12 the computer files that record the water quality 13 information. The first date would be the date I 14 believe a week after we sent them out and then the 15 last -- the last -- excuse me -- the last date would 16 be the day we picked them up. To the best of my 17 knowledge, anyways. 18 Q. I'd like you to turn now to page 33 in your 19 report. It's a pie chart. Figure 11, pie chart for 20 lack of a better term -- series of pie charts. 21 I'd like to call your attention to the 22 following sample sites, B-3, B-4, B-5, B-7. 23 A. On 33? 24 Q. Yes. 25 A. Okay. 79 1 Q. And 217. 2 A. Okay. 3 Q. Now, your total dissolved phosphorus for 4 B-3 was I think 39 parts per billion, is that 5 correct? 6 A. I don't know. I can't remember. Bear with 7 me for a minute. We can definitely relate this chart 8 to another table. Table A-5 and A-6. Table A-6, 9 actually. 10 Q. Okay. For B-3 the total phosphorus is 11 reflected to be 39 parts per billion on A-6, would 12 that be correct? 13 A. I don't know. For B-3? 14 Q. Yes. 15 A. Okay. Yes. 16 Q. Is that correct? 17 A. Right. 18 Q. And for B-4 it would be 18 parts per 19 billion? 20 A. Okay. Yes. That's the range obviously. 21 Q. Okay. B-5 is 5 parts per billion? 22 A. Right. 23 Q. And B-7 is 5 parts per billion? 24 A. Right. 25 Q. And 217 is 3 parts per billion? 80 1 A. Right. 2 Q. Now, refer back to Figure 11. 3 A. Okay. 4 Q. B-3 reflects species composition as being 5 primarily blue-greens? 6 A. Okay. 7 Q. Is that correct? 8 A. Okay. 9 Q. In fact, 79.2 percent -- 10 A. Okay. 11 Q. -- being blue-greens. 12 B-4 reflects species composition as being 13 primarily greens, 62.3 percent, is that correct? 14 A. Okay. 15 Q. B-5, again, primary composition being 16 greens with 66.2 percent? 17 A. Okay. 18 Q. And B-7 being primarily blue-greens at 63.1 19 percent? 20 A. Okay. 21 Q. And then 217 being primarily blue-greens at 22 68.5 percent? 23 A. Okay. 24 Q. Why did you find blue-greens primarily at 25 B-3 and B-7 which reflected quite different total 81 1 dissolved phosphorus concentrations but not at, for 2 example, at B-4? 3 A. I could answer that. This report I guess 4 was written before there was a great, you know, 5 debate, I guess, about the Everglades and all that it 6 simply does is take blue-greens, blue-greens whether 7 they're good, whether they're bad, whether they 8 represent a background site or whether they represent 9 a nutrient enriched site. B-3 -- and I'm getting -- 10 I'd have to go back and look at this stuff and we 11 probably will do that in a second -- but I would 12 assume that would be one species of possibly 13 Microcoleus spp. The background sites, for example, 14 are blue-greens, Schizothrix calcicola, Scytonema 15 hofmannii. Those are also blue-greens. They are 16 different in nature. The Microcoleus spp. being one 17 that we find. At least in the results of our 18 studies, found them in nutrient enriched situations. 19 They had high phosphorus content. We looked at 20 Scytonema hofmannii and Schizothrix calcicola and 21 those mats that we found at background sites were 22 very low nutrient content. Algae represented 23 primarily low nutrient alkaline hard water sites and 24 we just were simply just doing simple groupings of 25 the algae by major divisions and making no 82 1 distinction whether a blue-green is good, bad or 2 indifferent. These were the groups. 3 Q. Is there any way to parse out at this late 4 date what the relative composition was of the bad 5 blue-greens versus the good blue-greens? 6 A. That's your word, right? 7 Q. Well, you used it too, so -- 8 A. Yes. On page A-13 it breaks it out in B-3 9 Microcoleus spp. is the dominant species. You'll 10 find Schizothrix calcicola represented in maybe six 11 percent of that population, but you look over at the 12 B-7 and you'll find, for example, Schizothrix 13 calcicola representing a large portion of it. What 14 is missing in here is the 217 gauge. I can't explain 15 that. 16 Q. Is there any way to obtain any information 17 about what the 217 gauge was? 18 A. Not from this report, no. 19 Q. How would one go about obtaining that 20 information if it still exists? 21 A. Well, that is a good question. We have a -- 22 when I left Environmental Sciences in 1988 my 23 technician Brett Nicholas had a file that contained 24 all the raw data sheets. We would have a raw data 25 sheet of these -- the common species and their cell 83 1 volumes and so forth, so on and that was contained in 2 a folder along with water chemistry log sheets and 3 also the other stuff, so -- I do not know if that 4 stuff is existing now or not. 5 (Thereupon, a recess was taken.) 6 BY MR. HYDE: 7 Q. Let me ask you one final question, 8 Mr. Smith, (sic) about Exhibit 2. 9 When you identified the type of vegetative 10 cover at your sampling sites, did you or your team 11 attempt to quantify the relative percentages of the 12 vegetative composition at that site? 13 A. Not by a quantitative means like plots or 14 anything like that or transects. We just generally 15 took a look at the dominant vegetation. 16 Q. Okay. 17 A. That was not the purpose of the study. It 18 was just to get a general idea of what was there. 19 MR. HYDE: Could you label this Exhibit 3. 20 (The document was marked 21 Exb. No. 3.) 22 BY MR. HYDE: 23 Q. I'd like you to identify a document that's 24 been labeled Exhibit 3. 25 A. Exhibit 3 is a District Technical 84 1 Publication, 87-2 Periphyton and Water Quality 2 Relationships in the Everglades Water Conversation 3 Areas, 1978 through 1982. 4 Q. That document indicates that it's a draft. 5 Does that document before you differ from what 6 actually became the District Technical Publication 7 with the usual cover that one finds in those 8 documents? 9 A. Couldn't tell. 10 Q. Okay. 11 A. It's been so long ago I can't remember how 12 many changes were made or what changes were made. 13 Q. Okay. Would you anticipate there being any 14 significant changes between the two documents? 15 A. Um, no. As far as the -- as I recall, the 16 thrust of it was trying to show the differences in 17 community structure, biomass and its relationship to 18 water quality. That's the same theme, general theme, 19 I think. We sent this off to a number of outside 20 people for review. We got some comments back from 21 them. 22 Q. I'd like to draw your attention first to 23 page 14 of this document. 24 First of all, let me ask whether this 25 document was ever published in a peer review 85 1 scientific periodical or literature? 2 A. Um, no. I don't believe. The -- well, 3 let's see. I think a summary of this -- and I could 4 be wrong. I'm a little confused of which document. 5 In 1984 I had a document published in Environments of 6 South Florida and I think parts of this showed up in 7 there; not all of it, but just parts. 8 Q. I think maybe we should take a break and 9 not do this document at this time. It does appear to 10 significantly differ from the final draft at least in 11 its pagination. Maybe we could get a copy made of 12 this. 13 (Discussion held off the record.) 14 MR. HYDE: Let's postpone the discussion of 15 this document for the time being. 16 Label that Exhibit 4, please. 17 (The document was marked 18 Exb. No. 4.) 19 BY MR. HYDE: 20 Q. I'd like you to identify what's Exhibit 4. 21 A. Periphyton in the Everglades: Spatial 22 Variation, Environmental Correlates and Ecological 23 Implications by Joan Browder, Patrick Gleason, David 24 Swift. 25 Q. I take it you were a coauthor of this 86 1 study. 2 A. My name is on it, yes. Did I write it? 3 No. 4 Q. Okay. You were not the primary author of 5 it, then, I take it. 6 A. No. 7 Q. That was Miss Browder? 8 A. Yes. 9 Q. Okay. Do you know what relative 10 contribution Mr. Gleason had to it? 11 A. Well, I think he's in the same boat that I 12 was in. I basically provided copies of my technical 13 publications to Miss Browder and she attempted to, I 14 guess, synthesize the information. Patrick Gleason's 15 data is quite voluminous and I think she tried to 16 synthesize that and also my stuff and also basically 17 everybody else who has done any periphyton work in 18 South Florida, at least, and I really did not have 19 any direct influence in the paper. She asked me to 20 review it once. I reviewed it very quickly and I 21 said it's way too long and I haven't scene it really 22 since then. 23 Q. Okay. Do you agree with or stand by the 24 conclusions that are expressed in this paper? 25 A. I don't even know what the conclusions are. 87 1 Q. Okay. 2 A. If you quiz me on it, I couldn't really get 3 at it. 4 Q. So -- 5 A. I could -- I should myself indicate why we 6 got involved with this. This was part of the 7 Everglades Symposium and there were a number of 8 people who were trying to synthesize the existing 9 information on the Everglades and I was told to 10 basically contribute what I could to Dr. Browder to, 11 you know, synthesize the information together. 12 Q. Will you be offering any opinions at the 13 final hearing in this matter which are based on this 14 paper? 15 A. On the conclusions in this paper? 16 Q. Yes. 17 A. Not that I know of. 18 Q. Okay. Let me ask you a few questions about 19 it anyway. 20 A. Okay. 21 Q. I reviewed it. I feel like I have to. 22 Please refer to the second page, the 23 abstract portion of this paper, bottom of the page. 24 The paragraph reads, "Water quality at various 25 Everglades locations is determined largely by whether 88 1 the main source of surface water is local rainfall or 2 canal discharge. Hydroperiod and water depth at many 3 Everglades locations has been changed by canals, 4 levees, and other water control structures. Because 5 they affect both water quality and hydrologic 6 conditions, water management activities influence the 7 spatial distributions of the various periphyton 8 communities." 9 Do you agree or disagree with that 10 observation? 11 A. In part I agree with it. 12 Q. What part do you agree with first? 13 A. Water management activities do have an 14 influence on spatial distribution of periphyton and I 15 think she's trying to say hydroperiod and water depth 16 have been changed by canals. I agree with that. 17 (Ms. Stinson entered.) 18 BY MR. HYDE: 19 Q. What do you disagree with? 20 A. As far as the water management activities 21 it's very inclusive. I think you could be managing 22 water to, you know, to restore hydroperiods or to 23 store water in the system. Water management 24 activities could be, you know, taking stormwater 25 runoff from an area and moving it into or out of the 89 1 Everglades. I guess what I'm trying to get at, water 2 management activities is very general. I -- I'm not 3 exactly sure what she means by all of that. 4 Q. Okay. 5 A. It's -- there's a large number of things 6 that could be included. I think it's too inclusive. 7 Q. Let me ask the following question: How, by 8 your mind's light, do hydrologic conditions influence 9 spatial distributions in periphyton communities? 10 A. In Dr. Browder's studies of Everglades 11 National Park, she was looking at -- she found, I 12 believe, hydroperiod to be a primary factor or 13 gradient, I guess, in her studies and primarily, for 14 example, there's a disparity whether -- I think my 15 information show that major ions and water quality 16 was a major factor. In her study, if you look at 17 water quality differences between sites, they are 18 very little. Most of them are alkaline hard water 19 sites low in nutrients. The main or environmental 20 gradient that she was looking at would be differences 21 in water depth which relate back to hydroperiod. In 22 other words, whether periphyton developed in a deep 23 water slough versus a shallow water slough. That's 24 why, from her analysis of hydroperiod, it became an 25 important parameter in her analyses. 90 1 Q. Okay. If you disagree with Dr. Browder, at 2 least in this regard, why did you lend your name to 3 this publication? 4 A. Because I was told to lend my name to this 5 publication. I don't have a real big problem with 6 it. Just from the editorial standpoint, I -- this -- 7 I would have changed it. 8 Q. The next phrase is, "Dissolved oxygen 9 studies and diet studies with small aquatic animals 10 suggest that changes in the periphyton community 11 caused by either nutrient enrichment or shortened 12 hydroperiod may reduce habitat quality and carrying 13 capacity." 14 How does a shortened hydroperiod reduce 15 habitat quality and carrying capacity if you know? 16 A. That's basically from Joan's studies and I 17 think you'd have to ask Miss Browder. I believe 18 there was a study by someone by the name of Pope, I 19 believe, who was the gentleman who did it and he was 20 working for Joan as she was doing this Everglades 21 periphyton biomass study that she was working on. 22 Q. Do you intend to offer any opinions in that 23 regard? 24 A. No, sir. 25 Q. Do you claim any expertise in that regard? 91 1 A. Dissolved oxygen studies? May or may not 2 talk a little bit about dissolved oxygen, possibly. 3 Diet studies I would not be. I would rely on Miss 4 Browder's testimony if she was to be called. I don't 5 know that we plan doing that. 6 Q. Well, what would your opinion be as to the 7 effect of dissolved oxygen on shortened hydroperiod 8 and thereby reducing habitat quality and carrying 9 capacity? 10 A. Well, I think more of it is more related to 11 nutrient enrichment rather than shortened 12 hydroperiod. In other words, the dissolved oxygen 13 parameters that we've taken a look at are more 14 related to water quality and differences in nutrient 15 concentrations. 16 Q. Well, how does hydroperiod affect dissolved 17 oxygen concentrations? 18 A. I have no idea as far as what she's talking 19 about there. 20 Q. Okay. Do you intend to offer any opinions 21 as to how dissolved oxygen concentrations are 22 affected by hydroperiod? 23 A. No. 24 Q. The next phrase is, "In restoration 25 efforts, periphyton communities can be used as 92 1 sensitive site-specific indicators of environmental 2 quality." 3 Do you agree or disagree with that 4 statement? 5 A. I would say, yes, I would agree. 6 Q. Okay. How can periphyton communities be 7 utilized as such indicators of environmental quality? 8 A. In two areas. Number one, results of our 9 studies found that the nutrient content of the 10 periphyton community is -- tells us a good deal about 11 the history of water quality to conditions at those 12 sites. In other words, areas that had been exposed 13 or received a slug of nutrient enriched water, this -- 14 a lot of this is very quickly stored in that 15 periphyton mat and by simply going out and measuring 16 the phosphorus content of that mat, we get a good 17 idea of what the historical nutrient conditions have 18 been at that site. In the other regard, since the 19 1950s, periphyton communities have been used as 20 sensitive indicators of environmental quality. Going 21 back to Europe, a guy by the name of Fernstead -- 22 Hustead, (phonetic) those kinds of investigators 23 looked at and used diatoms, for example, as 24 indicators of water quality. In this country it's 25 been Ruth Patrick, a friend of mine; Charles Reimer 93 1 and several people at the Philadelphia Academy of 2 Sciences have developed the periphyton as a water 3 quality indicator. As you know, EPA uses this as a 4 bio monitoring tool in their surveillance studies of 5 impacts of water quality on aquatic communities and 6 it's really world wide. It's used in Czechoslovakia; 7 used in Russia; used it in Africa and Greece. This 8 is nothing new. It's been used. Periphyton are good 9 indicators of water quality. 10 Q. How quickly are -- is a slug of nutrients 11 taken up by periphyton? 12 A. Don't know that. We do know, based on our 13 studies, that relationship with water quality is at a 14 particular site and what the nutrient content of the 15 water was related back to the phosphorus content by 16 just some very crude looks at relationships between 17 the nutrient content and the surface water nutrient 18 chemistry. There's a very high correlation between 19 the two. As far as the length of time, it's hard to 20 say, but we're talking about the length of time that 21 a mat would be produced and would store the excess 22 phosphorus in that system. You can pick that up. 23 Q. Let me see if I can ask the question this 24 way. How far would nutrient enriched water move 25 prior to being taken up by periphyton? 94 1 A. Don't know. All I know is that a 2 relationship between those two is that the nutrient 3 content of the periphyton is very directly related to 4 surface water phosphorus content. 5 Q. Do you intend to offer any opinions as to 6 whether shifts in periphyton communities or break up 7 of periphyton mat are having some adverse impacts on 8 higher trophic species? 9 A. No, sir. 10 Q. That was a good answer. You saved all of 11 us a lot of time. 12 That's all I have of that document. 13 MR. HYDE: Label this Exhibit 5, please. 14 (The document was marked 15 Exb. No. 5.) 16 BY MR. HYDE: 17 Q. I'd like you to identify what's been 18 labeled as Exhibit 5. 19 A. Ecological Indicators of Water Quality In 20 the Wetlands of Southern Florida, David R. Swift, 21 Herbert Grimshaw, South Florida Water Management 22 District. 23 Q. Okay. What was this abstract prepared for? 24 A. Um -- 25 Q. Let me see if I can reflect your 95 1 recollection or refresh your recollection. 2 Was it presented to an International 3 Symposium on Ecological Indicators on Miami Beach? 4 A. Right. 5 Q. The second paragraph reads, "Statistical 6 analysis of these data revealed a highly significant 7 correlation between the percentage of cellular 8 phosphorus in periphyton and the concentration of 9 phosphorus dissolved in ambient marsh water." Citing 10 Swift 1981. 11 Was that the document that we were 12 referring to earlier which had been labeled Exhibit 13 2? 14 A. Um, probably is a misreference. I think it 15 should be also Swift and Nicholas 1987. 16 Q. Okay. 17 A. Those two documents, yes. 18 Q. So it should be both of those documents? 19 A. I would say so, yes. 20 Q. The next phrase reads, "Recent 21 reexamination of these data revealed a substantial 22 increase in the magnitude of this correlation 23 coefficient when data were grouped by location prior 24 to the statistical analysis." 25 Was this recent reexamination conducted by 96 1 both you and Mr. Grimshaw? 2 A. No. I basically turned over all the 3 nutrient chemistry due to phosphorus, cellular 4 phosphorus data over to Jim and he basically took 5 that information and reevaluated it and I really 6 didn't have anything to do with the data analysis or 7 its interpretation. It was done strictly by Jim. 8 Jim -- well, I think I'm wrong here. I'm talking 9 about two different things here. In this one, I 10 believe that Jim and I did collaborate on this one, 11 just looking at some of the raw data that we had 12 taken a look at and the reason I'm making the 13 distinction, I think Jim has another paper that he's 14 recently published or attempted to publish that I did 15 not have much input on. This one we did very quickly 16 take a look at some of the information for this 17 particular symposium, I guess, and presented that. 18 Q. Do you recall the statistical procedures 19 you went through to arrive at the conclusions 20 expressed in this paragraph? 21 A. Not exactly. 22 Q. Okay. 23 A. I'd have to go back and take a look at what 24 we did do. 25 Q. Do you know whether Mr. Grimshaw would 97 1 recall that? 2 A. Get him to testify, maybe he would. 3 (The document was marked 4 Exb. No. 6.) 5 BY MR. HYDE: 6 Q. I'd like you to identify what is Exhibit 6. 7 A. Says, "Abstract presented at the 14th 8 Annual Conference on Wetlands Restoration and 9 Creation May 1987." 10 Q. Okay. And on the second page of this 11 document is that your abstract that you prepared? 12 A. Right. 13 Q. Are the documents that attest to this, for 14 lack of a better term, overhead documents that you 15 utilized in your presentation? 16 A. I don't think so. I believe these were put 17 together as part of Walt Dineen's overheads that he 18 was putting together for the Governing Board. Some 19 of them may or may not have been used in the 20 presentation. I remember the presentation being 21 extremely short and I couldn't have possibly gone 22 over this, but I think what happened this might have 23 been -- I don't know why there -- they are stapled 24 together. 25 Q. Okay. Just the way it came to me to. 98 1 A. Okay. I think it's just a number of 2 overheads that had been used in presenting some data 3 to the Governing Board, I think. 4 Q. Okay. Well, let's just refer to your 5 portion of this document, the abstract which is page 6 2 -- 7 A. All right. 8 Q. -- the second page. 9 A. Uh huh. 10 Q. In the first paragraph you state, "Attached 11 algal (periphyton) communities, a major component of 12 the Everglades food chain, were monitored over an 13 eight year period (1978-1986) to assess the effects 14 of nutrient enrichment on the ecology of the 15 Everglades Water Conservation Area marsh." 16 Why do you assert here that algal 17 periphyton communities are a major component of the 18 Everglades food chain? 19 A. Well, I do not have a scientific 20 information that breaks down to what are the major 21 components, what are not the major components. It -- 22 the extent, for example, of the aquatic communities 23 in the Everglades is certainly obvious that the 24 periphyton are a large component of the biomass -- 25 the living biomass of the Everglades. As I said, 99 1 other people have found that the periphyton represent 2 a component of that food chain. Whether it's -- you 3 know, you can scientifically document that it's the 4 major component, I don't believe anybody has done 5 that yet. But I do believe that it is an important 6 component of the system and a major component of the 7 system. Just from the magnitude of biomass of carbon 8 produced it would be obvious that it would be 9 difficult to overlook it as a contributor. 10 Q. I think maybe I heard two things there. 11 Were you saying that periphyton is a major component 12 of the Everglades system? 13 A. Uh huh. 14 Q. Or major component of the Everglades food 15 chain? 16 A. Of the system, right. 17 Q. I thought you also -- 18 A. Exact role in the food chain has not been 19 determined as of yet. 20 Q. Okay. What is your basis for saying that 21 periphyton is a major component of the Everglades 22 system? 23 A. Just from the standpoint of you have your 24 aquatic vascular plants and then you have vast acres 25 and acres and acres of algal mats that are present in 100 1 the system. In Everglades National Park those algal 2 mats can be from three to six inches thick covering 3 miles and miles and miles of marsh. 4 Q. Are you essentially saying that basically 5 because there's a lot of it there, it has to be 6 significant? 7 A. Well, you are saying it's a component. 8 Q. Okay. Well, I think you said it was a 9 major component of that system. 10 A. Right. 11 Q. Is that -- is the significance of the 12 periphyton simply its geographic spread that makes it 13 a major component? 14 A. It represents a large portion of the living 15 biomass of the system. It's unlikely that that 16 living biomass is not utilized for something. We 17 don't know exactly what it -- what it is utilized 18 for; specifically how much energy is derived from 19 periphyton; how much energy is derived from detrital 20 components of the -- of sawgrass or other components 21 of macrophytes. It hasn't been determined yet. 22 (Ms. Stinson left.) 23 BY MR. HYDE: 24 Q. What happens to the periphyton in drought 25 conditions? 101 1 A. It desiccates, falls back to the bottom of 2 the marsh. You can -- many times the calcium 3 carbonate component shows up kind of -- if you flew 4 over the area it looks like a grayish white and that 5 represents the desiccated algal mats. 6 Q. Have you made any effort to quantify that 7 periphyton biomass that you were referring to? 8 A. When we first got into the study, that was 9 one of the things we were interested in, but since we 10 were trying to relate it, compare apples with apples, 11 it was very difficult because the hydroperiod, the 12 amount of time, for example, that water was present 13 at a site, we thought that would have a major 14 significance of how much -- how thick an algal mat 15 was or how much algae could be produced at a given 16 site and we wanted to make sure that we were looking 17 at one thing and that's water quality. This 18 particular study, at least we think, is the only one -- 19 only study that exists that tries to rule out the 20 effects of hydroperiod by putting out glass slide 21 substrates to look at growth rates, to look at growth 22 of algae on slides and just to look at the water 23 quality component of the study and that's the -- at 24 least that's what we designed it for. That's why, 25 trying to get int