834 1 DIVISION OF ADMINISTRATIVE HEARINGS DEPARTMENT OF ADMINISTRATION, STATE OF FLORIDA 2 3 SUGAR CANE GROWERS COOPERATIVE ) OF FLORIDA; ROTH FARMS, INC., and ) 4 WEDGWORTH FARMS, INC., ) Petitioners, ) DOAH Case No. 92-3038 5 v. ) SOUTH FLORIDA WATER MANAGEMENT ) 6 DISTRICT, an agency of the State ) of Florida; et al., ) 7 Respondents. ) - - - - - - - - - - - - - - - - - - x 8 FLORIDA SUGAR CANE LEAGUE, INC.; ) UNITED STATES SUGAR CORPORATION; ) 9 and NEW HOPE SOUTH, INC., ) Petitioners, ) 10 v. ) DOAH Case No. 92-3039 SOUTH FLORIDA WATER MANAGEMENT ) 11 DISTRICT, an agency of the State ) of Florida; et al., ) 12 Respondents. ) - - - - - - - - - - - - - - - - - - x 13 FLORIDA FRUIT AND VEGETABLE ) ASSOCIATION; LEWIS POPE FARMS; ) 14 W.E. SCHLECHTER & SONS, INC., ) and HUNDLEY FARMS, INC., ) 15 Petitioners, ) v. ) DOAH Case No. 92-3040 16 SOUTH FLORIDA WATER MANAGEMENT ) DISTRICT, an agency of the State ) 17 of Florida; et al., ) Respondents. ) 18 - - - - - - - - - - - - - - - - - - x 100 S.E. 2nd Street 19 Miami, Florida April 6, 1994 20 10:10 a.m - 5:50 p.m. 21 DEPOSITION OF RONALD D. JONES 22 Taken before THOMAS R. NEUMANN, Registered Professional Reporter and Notary Public in and for 23 the State of Florida at Large, pursuant to Notice of Taking Deposition filed in the above cause. 24 - - - - - - - 835 1 APPEARANCES 2 ON BEHALF OF THE PETITIONERS FLORIDA SUGAR CANE 3 LEAGUE, INC., UNITED STATES SUGAR CORP., and NEW SOUTH HOPE, INC. 4 EARL, BLANK, KAVANAUGH & STOTTS, P.A. 5 215 So. Monroe Street Suite 350 6 Tallahassee, Florida 32301 BY: WILLIAM L. HYDE, ESQ. 7 ON BEHALF OF THE RESPONDENT-INTERVENOR 8 UNITED STATES OF AMERICA 9 SUZAN HILL PONZOLI, ESQ. ASSISTANT U.S. ATTORNEY 10 99 N.E. 4th Street Miami, Florida 33132 11 ON BEHALF OF THE PETITIONERS SUGAR CANE GROWERS 12 COOPERATIVE OF FLORIDA, ROTH FARMS, INC., AND WEDGWORTH FARMS, INC. 13 HOPPING, BOYD, GREEN & SAMS 14 123 South Calhoun Street P.O. Box 6526, 15 Tallahassee, Florida 32314 BY: GARY P. SAMS, ESQ. 16 INDEX 17 Witness Direct Cross Redirect Recross RONALD D. JONES 18 By Mr. Hyde: 837 947 By Mr. Sams: 892 836 1 2 EXHIBITS 3 NUMBER BATES NO. PAGE 4 Composite Exhibit 89.............................893 Exhibit 90.......................................901 5 Exhibit 91.......................................910 Exhibit 92.......................................910 6 Exhibit 93.......................................914 Exhibit 94.......................................916 7 Exhibit 95.......................................922 Exhibit 96.......................................926 8 Composite Exhibit 97.............................929 Exhibit 98.......................................947 9 Exhibits 99 through 109..........................948 Composite Exhibit 110............................971 10 Exhibit 111......................................974 Exhibit 112......................................977 11 Exhibit 113......................................988 Exhibit 114......................................990 12 Exhibit 115......................................991 Exhibit 116......................................992 13 Exhibit 117......................................998 Exhibit 118......................................999 14 Exhibit 119.....................................1000 Exhibit 120.....................................1007 15 Exhibit 121.....................................1008 Exhibit 122.....................................1009 16 Exhibit 123.....................................1010 Exhibit 124.....................................1010 17 Exhibit 125.....................................1011 Exhibit 126.....................................1011 18 837 1 Thereupon -- 2 RONALD D. JONES 3 was called as a witness and, having been first duly 4 sworn, was examined and testified as follows: 5 DIRECT EXAMINATION 6 BY MR. HYDE: 7 Q. Just again for purposes of the record would 8 you please state your name and address, please? 9 A. My name is Ronald D. Jones. I reside at 10 15069 Southwest 13th Court, Sunrise, Florida, 33190. 11 Q. Dr. Jones, as you well know my name is 12 William Hyde. I'm with the firm of Earl, Blank, 13 Kavanaugh & Stotts. 14 I'm here to depose you today in a follow up 15 deposition concerning your anticipated testimony in 16 the so-called Everglades SWIM Plan proceeding which 17 may or may not occur, depending on what the Florida 18 legislature does in the next few days. 19 MS. PONZOLI: Or hours. 20 MR. HYDE: My primary purpose here today is 21 to find out as much as I can about your data 22 collection and analytical tasks, especially as 23 they relate to phosphorous in the Everglades 24 Protection Area. 25 And just before I get into that particular 838 1 category of questioning, I wanted to just do a 2 couple of clean up questions from our previous 3 deposition. 4 BY MR. HYDE: 5 Q. During our previous deposition we 6 identified a series of documents. I think they were 7 your declaration from the federal litigation in 8 support of the U.S. motion for summary judgment and 9 then another document entitled "Supplemental 10 Testimony of Ron Jones." They were Exhibits 53 and 11 55 where you, in effect, expressed various opinions 12 about what your testimony would be in the context of 13 a hearing as to adverse impacts on the Everglades. 14 Do you intend at this moment or at this 15 time to offer any opinions in addition to those that 16 are set forth there? And you can take a look at 17 Exhibits 53 and 55 if you would like to refresh your 18 recollection. 19 MS. PONZOLI: Do you have them with you? 20 MR. HYDE: Yes, they are a faxed copy. 21 MS. PONZOLI: I do want to indicate that I 22 believe the United States had indicated at the 23 other deposition -- the beginning of this 24 deposition that Dr. Jones will also offer 25 testimony on phosphorous cycling, which he was 839 1 questioned for several days by the cooperative. 2 We don't anticipate at this time that we will be 3 putting on an affirmative case, but we 4 anticipate he would be offered in rebuttal 5 testimony in that regard. 6 I think we had also indicated that 7 Dr. Jones would give expert testimony on the use 8 of chemical treatment for phosphorous removal. 9 To the best of my recollection, those are the 10 only two sort of generic additions that we 11 anticipate his adding. 12 MR. HYDE: I thank you for clarifying. 13 Once you mentioned it, I did recall those two 14 myself. 15 BY MR. HYDE: 16 Q. Dr. Jones, I want you to take a look at the 17 document and make sure there was something that was 18 not being left out. 19 A. I might have a question concerning 55. And 20 that would be, is this the modified version because I 21 had not -- 22 Q. 54 was the earlier version which you did 23 modify. I thought it was attached. 24 MS. PONZOLI: Didn't we actually write on 25 this -- 54 would be ahead of this. 840 1 THE WITNESS: It's not it. 2 MR. HYDE: It should be there. 3 THE WITNESS: This is correct. I'm sorry. 4 I want to make sure we are looking at the same 5 one. 6 BY MR. HYDE: 7 Q. We are referring to Exhibits 53 and 55 from 8 the previous deposition? 9 A. That's correct. And it is my understanding 10 with the exception that counsel just made that this 11 is it, the area. 12 Q. Now onto the main purpose of the deposition 13 today. 14 Dr. Jones, I want to go through the general 15 areas where you have done sampling and analysis, 16 first just to make sure I covered them. Then we can 17 get into specific documents. 18 My review of your previous deposition and 19 the documents that we just referred to, Exhibits 53 20 and 55, indicate to me that you have done the 21 following. Please note this is my assessment. There 22 may be additional stuff. 23 You measured whether phosphorous has 24 accumulated in the soils of the Park and the refuge; 25 correct? 841 1 A. That's correct. 2 Q. You measured the areal phosphorous 3 concentrations in the soils along transects in the 4 Park, the Loxahatchee refuge, Water Conservation Area 5 2A and Water Conservation Area 3A; is that correct? 6 A. That's correct. 7 Q. You also measured alkaline phosphatase 8 activity in the water at sampling points along a 9 transect in the Everglades National Park and in the 10 perimeter canal of the Loxahatchee refuge, correct? 11 A. That's correct. There were additional 12 places, also. 13 Q. What additional places did you measure 14 alkaline phosphatase? 15 A. In the water column across the transects in 16 Loxahatchee along the transects in Water Conservation 17 Area 3A and in the canals. I guess it would be in 18 the canal down along U.S. 41. 19 Q. You said also along transects in the refuge 20 and the canal along U.S. 41. Where else was it? 21 A. Water Conservation Area 2A and 3A. I'm 22 limiting it to work that's done in the Everglades 23 freshwater environment because we have a number of 24 things done in Florida Bay and Whitewater Bay, also. 25 I don't wanted you to think -- 842 1 Q. For purposes of my questioning here today 2 I'm only concerned about that which you have done in 3 the Everglades Protection Area, which would be 4 specifically the refuge, the Park, water conservation 5 areas 2A, 2B, 3A and 3B. I'm not concerned about 6 Florida Bay. 7 A. Florida Bay is part of the Everglades 8 Protection Area because it's within the Park. 9 Q. I understand that, but I'm not really 10 interested for purpose of my question in Florida Bay. 11 A. I understand. 12 Q. Unless for some reason I change my mind, 13 but it doesn't seem likely. 14 A. I understand. 15 Q. Have you collected any phosphorous related 16 data, either soil, water in water conservation areas 17 2A, 2B or 3 or 3B? 18 A. I have some information for 3B. I don't 19 believe I ever collected any samples in 2B. I have 20 been there and I may have collected some samples and 21 had them dried and they would be in storage, but they 22 have not been analyzed -- most certainly have not 23 been analyzed. 24 Q. For what purpose did you collect data in 25 3B? 843 1 A. I collected samples in 3B. I would say 2 that samples and data are different from that 3 standpoint. 4 Q. Well, as modified, what did you do in 3B? 5 A. I collected samples near the Richardson 6 sites, if memory serves me. 7 Q. You say Richardson. You are referring to 8 Dr. Curtis Richardson? 9 A. I'm sorry, I'm referring to 2B. I'm sorry. 10 Curtis Richardson sites in 2B. Your question was for 11 3B, I apologize. 12 Q. Just to make sure I understand, you 13 collected some data or you did some sampling in 2B? 14 A. That's correct. 15 MS. PONZOLI: Off the record. 16 (Discussion off the record.) 17 BY MR. HYDE: 18 Q. Why did you collect the samples in 2B from 19 the, I presume, the Duke Wetland Center sites? 20 A. Near the Duke Wetland Center sites. They 21 were collected just to get an idea perhaps of what 22 the total phosphorous content of the soil was in 23 those areas if there was reason to believe that the 24 data in the Duke wetland report was incorrect or for 25 comparison purposes. 844 1 Q. Did you find anything that indicated that 2 the Duke Wetland Center data was incorrect or 3 misleading? 4 MS. PONZOLI: Object to the form. I think 5 that's a pretty broad question. I don't know he 6 has even seen all of the Duke wetland site data. 7 MR. HYDE: I'm referring in the context of 8 this particular sampling effort for 2B. 9 THE WITNESS: I have not gone in any 10 further to actually do that. Time has been 11 pretty tight, so I still have those samples. 12 They may be analyzed in the future, but I doubt 13 it. 14 BY MR. HYDE: 15 Q. Do you anticipate you will do that before 16 trial in this cause? 17 A. I don't know. I doubt it. 18 Q. Excluding the Everglades agricultural area, 19 have you collected any other data including but not 20 necessarily limited to phosphorous data, either water 21 or soil and alkaline phosphatase data in the 22 Everglades Protection Area other than what you have 23 just noted for me here today? 24 A. Yes. 25 Q. What other data have you collected? 845 1 A. The data -- I have a student who is working 2 in Water Conservation Area 3A. We collected both 3 mercury and phosphorous data in a grid network or 4 grid pattern in the southern portion of Water 5 Conservation Area 3A. 6 Q. Was that total phosphorous data? 7 A. Total phosphorous in the soil and total 8 mercury in the soil. 9 I have collected samples along with the 10 Environmental Protection Agency for mercury and 11 phosphorous in the canals, 50 canal stations. 12 Q. Again, was that total phosphorous? 13 A. Total phosphorous in soil and water 14 sediment and water and mercury. 15 Q. At 50? 16 A. 50 canal stations, exactly. 17 Q. That's throughout the EPA? 18 A. Well, that's all the way from Lake 19 Okeechobee to Florida Bay. 20 Q. Have you collected any other data? 21 A. Yes, at various projects throughout the 22 last ten years there have been samples that have been 23 collected that have phosphorous values or alkaline 24 phosphatase values associated with them, various 25 other parameters. 846 1 Q. How does that data differ from that which 2 you have already identified? 3 MS. PONZOLI: Object to the form. 4 THE WITNESS: I'm sorry. I don't 5 understand what you mean. 6 BY MR. HYDE: 7 Q. Well, you indicated, for example, that you 8 had measured alkaline phosphatase activity in the 9 Park and in the perimeter canal of the refuge and 10 also along some transects in the refuge canal along 11 U.S. 41 and WCA-2A and 3A. Just a moment ago you 12 indicated that you had done some additional alkaline 13 phosphatase measurements. 14 I'm trying to understand how this latest 15 category is different from the -- more segregated 16 from what you had previously identified. 17 A. The question I believe, unless I really 18 misunderstood it, was total phosphorous, other 19 analysis -- not alkaline phosphatase. 20 I have not necessarily measured alkaline 21 phosphatase at all of those other additional 22 stations. There was additional alkaline phosphatase 23 work done. It was not such a regular or in a defined 24 pattern as the transect studies were. 25 In addition, there was a document produced 847 1 at my last deposition which had a series of locations 2 where we measured alkaline phosphatase and total 3 phosphorous in the water column on a grid in 4 Everglades National Park in Water Conservation Area 5 3A. That document is missing with my earlier 6 production last year. And I would desperately like 7 to find that because it was representing a lot of 8 work with alkaline phosphatase. I can't go back and 9 repeat those -- I can't reanalyze the samples because 10 they were water and they had to be discarded. That 11 work exists. It was lost. 12 Q. So we don't have it either, correct? 13 A. I have not seen it in the documents. We 14 found the three or four pages of material that showed 15 where the sampling locations were -- had conductivity 16 measurements and a small map indicating where the 17 grid points were. What was attached to that was also 18 the data and the data is missing. 19 Q. Well, perhaps if we come across it we can 20 revisit it. 21 Is there any other data that you have 22 collected in the EPA -- excluding, of course, the 23 Everglades agricultural area and Florida Bay? 24 A. We have a number of laboratory studies that 25 have been done with samples collected from the EPA. 848 1 And I guess from that standpoint it's not field data, 2 but it is using samples from the area. 3 Q. What were the purpose or purposes of the 4 lab studies? 5 A. There were two major premises. One was to 6 examine the uptake and incorporation of phosphorous 7 removal efficiencies, if you will, phosphorous from 8 the water column and its various forms by soils from 9 predominantly Everglades National Park which had 10 different levels of total phosphorous. 11 The other was to examine the effects that 12 phosphorous had on limiting various microbial 13 activities in the soil. 14 Q. Is there any other work that you have done 15 along these lines beyond that which you have already 16 identified? 17 A. I'm thinking. 18 Q. Take your time. 19 A. It has been ten years. 20 Q. I have trouble remembering what I did ten 21 weeks ago, so don't worry about it. 22 A. I have done some work in Loxahatchee 23 National Wildlife Refuge pertaining to total 24 phosphorous and alkaline phosphatase activity and 25 water column concentrations of total phosphorous, 849 1 orthophosphorous along gradients extending from tree 2 islands that had bird rookeries on them. 3 I have also analyzed a number of samples 4 from Loxahatchee National Wildlife Refuge pertaining 5 to total phosphorous and rainfall and total 6 phosphorous in the 16 stations or whatever that are 7 being used for monitoring in the refuge. And I have 8 analyzed a number of soil samples that were collected 9 in parallel with the League and the cooperative entry 10 and access into various areas. 11 Q. Is that all? 12 A. Let me take a moment. 13 I had a student who did work in the 14 Everglades National Park in Water Conservation Area 15 3B looking at methane production and how it related 16 to various other soil parameters. Total phosphorous 17 would have been one of those. 18 Q. This work was done in Everglades National 19 Park in Water Conservation Area 3 -- 20 A. 3B. 21 Q. -- and the purpose was to investigate 22 methane? 23 A. Methane production, methanogenesis. 24 Q. Why was that study being done, for what 25 purpose? 850 1 A. There was just a Master's degree student 2 paper. I worked for many years now in the area of 3 trace gas chemistry, analytical chemistry. It is one 4 of my interests, and so it was not done for the 5 purpose of this issue. It was just something that 6 happened during that same time frame. 7 Q. When you say it was not done for the 8 purpose of this issue, do you mean for the purposes 9 of this proceeding? 10 A. Yes, that is correct. 11 Q. You are not going to be relying on it for 12 the purposes of your opinions? 13 A. No. I may very well rely on it. It 14 contains some valuable information. I can't discard 15 the stuff that I learned ten years ago just because 16 this proceeding started six years ago. 17 Q. I understand that. I'm just trying to find 18 out what you are relying on. 19 A. Yes. 20 Q. For what purpose would you rely on that 21 person's, I guess, Master's thesis? 22 A. Yes. 23 Q. Is it completed? 24 A. It's completed and the work was published. 25 Q. Who is the Master's student? 851 1 A. Dave Bachoon and myself. 2 Q. Do you recall in general terms what the 3 title of that paper was? 4 A. It had the word "methanogenesis." 5 "Potential Grades of Potential methanogenesis." You 6 would have been provided a copy. 7 Q. I think I do recognize it. 8 What important information did you glean 9 from that study for purposes of your opinion? 10 A. It provides more of a background 11 information. It provides me with some E, H or redox 12 potential of the soils within Everglades National 13 Park, and then just sort of general experience or 14 whatever in the Everglades that I have had. It's all 15 part of my background. 16 Q. What does it show you about redox 17 potential? 18 A. That the soils particularly in Everglades 19 National Park are not highly reduced, that there is 20 oxygen present throughout the soil column even though 21 the soils are flooded. 22 Q. Now we have gone through this methane 23 paper. Are there any other documents that you can 24 think of or studies or data that relate to Everglades 25 work? 852 1 A. There is current work that's taking place. 2 Q. What is your current work? 3 A. We are currently starting as of yesterday 4 and today and throughout next week -- the 5 Environmental Protection Agency is down and we are 6 running the transects in -- the four transects again. 7 We will be looking at mercury and phosphorous along 8 the transects. 9 Q. Is that for total phosphorous? 10 A. Total phosphorous and water in the soil. 11 Q. And mercury? 12 A. And mercury. 13 Q. Where are these transects? 14 A. The transects are the same transects. 15 Everglades National Park transects, Water 16 Conservation Area 3A transects, 2A transects and 17 Loxahatchee National Wildlife Refuge transects. 18 Q. Are you engaged in any other current work 19 in the Everglades Protection Area? 20 A. Yes. I have a student working on 21 Everglades National Park on nitrogen cycling. I have 22 a post doc working on phosphorous effects on various 23 microbial enzymatic processes, and there are two 24 students working on mercury in -- well, the Park and 25 in Water Conservation Area 3B. 853 1 Q. I believe you just stated this work is also 2 ongoing? 3 A. Yes. 4 Q. When do you anticipate it being done and 5 why don't you just break it down in terms of a 6 particular study being done? 7 A. The EPA mercury work I see going on for a 8 long time. You know, there will be obviously results 9 coming out throughout the course and publishing some 10 papers now or working getting some papers published 11 now. But I think that's probably over the next five 12 years. 13 Other projects will take between, you know, 14 one and two years. I can't really break them down. 15 There are always results coming in all the time. And 16 when the results are sufficient to publish or to 17 submit an article for publication, at that point the 18 project may not be complete, but at that point we 19 would submit an article for publication. 20 Q. Has any of this current work reached that 21 state? 22 A. Yes. We have two articles. The abstracts 23 have been accepted and we have been asked to write 24 articles for a mercury conference that's coming up 25 later on in the summer. And we are submitting a 854 1 paper which I produced last time for you all to look 2 at on the "Analytical Methodology for Mercury 3 Speciation Determinations." 4 There are a number of abstracts. I just 5 can't recall which ones they would be that are in 6 various stages of press or whatnot. 7 Q. Is all of this work concerned with mercury? 8 A. Mercury and phosphorous, both. With the 9 exception of the analytical paper, which is really 10 just a methods paper. 11 Q. The analytical paper is which one? 12 A. It would be of "Speciation of Mercury by 13 Gas Chromatography." 14 Q. Has any of this work specifically or solely 15 related to just phosphorous? 16 A. No. I take that back. 17 The one project that my post doc is doing 18 is very much a phosphorous project. But it has in 19 addition to that microbial activities, so it's not 20 just a measurement of phosphorous. 21 Q. Has that project matured to a state where 22 you have information that you can rely on for 23 purposes of your opinions here? 24 A. There is some information that has been 25 generated by that project which further substantiate 855 1 published works that we have already done with 2 microbial processes. It has not gone far enough 3 where we are ready to publish it. We are in the 4 process of working out the methodologies. 5 Q. How does it serve to substantiate those 6 earlier papers? 7 A. It indicates, as the earlier publications 8 have, that phosphorous is the key limiting factor in 9 the Everglades, and that the nutrient pollution 10 coming into Everglades National Park is altering 11 these activities in the soil. 12 Q. By "activities," you mean microbial 13 processes? 14 A. Microbial activities, correct. 15 Q. Is that post doctorate study available or 16 is the data available for it? 17 A. There is no data at this point. What we 18 have been doing is, we are shifting from using a 19 enzyme substrate that had ortho-methylfluorecene as 20 the indicator to an umbileferone. 21 It requires a lot of preliminary work to 22 make a change like that in the basic methodology, so 23 we are working those methods out. As we work the 24 methods out, we gain some insight because we are 25 using natural samples. But the data from that is not 856 1 collected in the manner or the same way as if we were 2 going to do these assays. 3 What we are doing is just finding out 4 whether these techniques work, how to make them work, 5 and then we will go on and do a very defined 6 experimental approach with a number of stations along 7 the transects. 8 Q. I believe you previously indicated that you 9 turned over, I guess, the bulk of this information 10 to -- perhaps all of it, but I want to make sure we 11 do have things, if you can just bear with me for a 12 moment because I'm not myself intimately familiar 13 with all of this. 14 You have turned over, I take it, the soil 15 phosphorous data that you have collected in the Park 16 refuge, water conservation areas 2A and 3A; correct? 17 A. With the exception of the documents. 18 MS. PONZOLI: These, the ones we brought 19 today, he has done some updating since his last 20 depo. 21 BY MR. HYDE: 22 Q. We will get to that. Beyond these 23 documents, which I would like to take a look at in a 24 while. 25 A. Beyond those, you have everything. 857 1 Everything was produced. Whether it was actually 2 selected and Xeroxed, I don't know. 3 Q. And that would be the same for the 4 phosphorous concentrations and the water columns in 5 those same areas? 6 A. Yes. 7 Q. That would also include your alkaline 8 phosphatase activity along the Park transect, 9 perimeter canal of the refuge, transects in the 10 refuge, canal on U.S. 41 and Water Conservation Area 11 2A and 3A? 12 A. Where the data is available it has been 13 provided for selection. I ought to point out that 14 these documents were not Xeroxed in totality. I 15 mean, you all selected what you wanted and I don't 16 know for sure what that was. 17 Q. Did you review the documents that we had 18 selected -- when I say "we," I mean what my firm 19 selected -- to ascertain whether documents had been 20 left out that related to those areas? 21 MS. PONZOLI: Object to the form. 22 THE WITNESS: I looked through the 23 documents. I recall there were something like 24 maybe two and a half, three banker's boxes of 25 selected documents. And obviously going through 858 1 that many pages of material, I couldn't be as 2 complete in the review. 3 I did note that there were certain 4 materials that were presented that were not in 5 the Xerox of selected documents. 6 BY MR. HYDE: 7 Q. Could you identify for me, if you can 8 recall, what some of these more obvious omissions 9 were? 10 A. File folders, the file folders with the 11 label of what was in them. 12 Q. You are talking about just the file folder 13 itself? 14 A. That contained the data with the label on 15 the top describing what it was. That often was not 16 included. A number of -- I'll call it machine output 17 or computer paper with just many, many numbers from 18 the autoanalyzer. 19 MS. PONZOLI: Being they took the summary 20 data but not the raw data? 21 THE WITNESS: That's correct. 22 BY MR. HYDE: 23 Q. Any other obvious omissions? 24 A. That's -- those are the two that I recall 25 just from going through the documents. 859 1 Q. You indicated that you had also collected 2 some data in Water Conservation Area 2B nearby the 3 Duke Wetland Center sites? 4 A. I collected samples, yes. 5 Q. You collected some samples near the Duke 6 Wetland Center sites in Water Conservation Area 2B; 7 is that correct? 8 A. That's correct. 9 Q. Was that turned over? 10 A. There is no data. There are little ground 11 up bottles of dirt and they are hard to Xerox. 12 Q. So it's just the samples, you haven't 13 performed any analysis? 14 A. No analysis. That's why I distinguished 15 between data and samples. 16 Q. What about the bulk mercury and total 17 phosphorous data from the soil in Water Conservation 18 Area 3A? 19 A. That data has been turned over with the 20 exception of what's contained in the new documents. 21 Q. Now, the mercury and total phosphorous, are 22 they just samples or data in soil and water from the 23 50 canal stations? 24 A. There is now data on the complete set of 25 data. 860 1 Q. You are saying there is now data. Is that 2 something to be turned over? 3 A. Yes, portions of it. Portions were 4 available the last time you deposed me. We indicated 5 this was an ongoing project. As that data becomes 6 available we would make it available. Last week I 7 received some information. 8 Q. I just want to make sure I understand what 9 it is. 10 A. Yes. 11 Q. The data from lab studies done on samples 12 from the EPA to examine uptake of phosphorous from 13 the water column -- I guess it was primarily from the 14 Park -- have to do with measuring the effects of 15 phosphorous and microbial activities? 16 A. You have the data and the published papers 17 for that, and the submitted articles that went along 18 with the data. 19 Q. Now, what about the total phosphorous and 20 alkaline phosphatase measurements along the gradients 21 extending from bird rookeries? 22 A. You have that data. 23 Q. Was that done in the refuge only? 24 A. Yes. 25 Q. What about the total phosphorous 861 1 measurements in rainfall and at 16 stations of the 2 refuge? 3 A. You have that data, and there is additional 4 data to be produced today. 5 Q. Now, what about data that had been 6 processed in connection with the, I guess, Master's 7 paper on methane production? 8 A. You have the paper and the whole file that 9 contains all of the data with that. 10 Again, I should say I produced that data. 11 I cannot -- considering the way it was done, the 12 whole packets weren't taken in always. I can't 13 remember how many purple stickies or orange stickies 14 were stuck on the things for FLSC. 15 Q. You did not withhold any of it? 16 A. No. I turned over everything that was in 17 my file with the exception of privileged information. 18 Q. Were your soil sediment samplings or 19 phosphorous all collected in the same manner? 20 A. No. 21 Q. Would you describe for me the different 22 ways -- first of all, how many different ways did you 23 collect soil samples for phosphorous? 24 A. Approximately five. 25 Q. Did you collect the samples in different 862 1 ways to learn different things, or was it just sort 2 of an evolution over time? 3 MS. PONZOLI: Object to the form. 4 THE WITNESS: Actually, the collection 5 method, a number of them are still being used. 6 Certain areas it's easier to collect it one way, 7 others areas it's impossible to collect it. 8 BY MR. HYDE: 9 Q. Why don't you take me down the list of the 10 different ways that you have collected soil sediment 11 for phosphorous analysis? 12 A. Okay. When we are sampling in canals or in 13 deeper water areas, we use what's referred to as an 14 Eckman core or box core sampler. 15 Q. Spell that. 16 A. E-C-K-M-A-N. There may not be a C in 17 there. It might be E-K-M-A-N. And that is, simply, 18 it looks like a clam shell. It goes down and sits on 19 the bottom and grabs a sediment sample. 20 We have also used what is called an 21 eggshell core device, and this is simply a piece of 22 stainless steel pipe with a polycarbonate plastic 23 liner in what's called an eggshell at the bottom of 24 it which is a very, very thin piece of plastic that 25 acts -- as a sample goes in, it looks like a series 863 1 of fingers that opens up. And then when you draw the 2 sample up, the fingers are forced back closed and it 3 retains the sample in the coring tube. 4 Q. In what area is the eggshell core device 5 utilized? 6 A. We used that in the canals. And we have 7 also utilized it in the -- just by hands in the water 8 conservation areas. 9 Q. What's the third category? 10 A. The third category would be PVC cores. 11 Q. And where was that? 12 A. That was predominantly used in the water 13 conservation areas and the Everglades National Park, 14 Loxahatchee. That was used in all of the areas. 15 Q. And then what's the fourth category? 16 A. The fourth category would be what we call a 17 grab sample, which is using a hand when you go out. 18 We used that sampling methodology everywhere. It's 19 probably the predominant mechanism that we used after 20 we compare -- we have used that for comparison with 21 cores. 22 We find out there is no difference between 23 cores and the grab sample. We used the grab sample 24 because it's much easier than trying to carry a 25 sampling device. 864 1 Q. And you indicated there was a fifth one? 2 A. Fifth and really another coring device. 3 It's a large polycarbonate tube and we use these 4 tubes when we want to make thin sections of the soil, 5 when we want to profile the soil, say, two centimeter 6 increments. Or if we want to get certain fractions, 7 we will use those cores. There are other mechanisms 8 that we have used, but they are all a variation on 9 these five principals on these devices. 10 Q. When you were sampling the water column for 11 phosphorous, did you use one or several different 12 methodologies for doing so? 13 A. I have used other methods, but 14 predominantly in the Everglades we simply submerged a 15 sample bottle. We have a protocol for how you go 16 about filling those bottles. 17 Q. What other methodologies did you utilize to 18 sample the water column? 19 A. We collected samples using syringes from 20 very shallow areas. We have also collected samples 21 from a pumping device. Pumping is more of a vacuum 22 suction device rather than pumping, and we collected 23 on some occasions from canals or bridges. At 24 structures like that we will use a polyethylene pail, 25 bucket, on a line. We also have a teflon coated 865 1 Niskin bottle. 2 Q. How do you spell that? 3 A. N-I-S-K-I-N. 4 Q. What is that? 5 A. It's an expensive sampling device designed 6 for oceanographic sampling. We have utilized that on 7 certain occasions. We generally reserve the use of 8 those types of bottles for oceanographic work. I 9 have used it in the marsh. 10 Q. Any other methodologies used? 11 A. No, it's all variations and the protocols 12 are various, depending on what kind of sample you are 13 going to collect. 14 Q. How would one know from looking at your 15 records that a particular sampling methodology was 16 employed? 17 A. For water? 18 Q. Just for water for the moment. 19 A. It would be described in the materials and 20 methods portion of the papers that are published, or 21 generally it's just knowledge. For instance, I know 22 how I collected the samples in the marsh. When I 23 would write a paper I would include that methodology. 24 We are now doing work for the state of 25 Florida and have a -- you have to have DEP approved 866 1 project plans for those particular types of work. 2 Under those circumstances, it's actually described in 3 the project plans. 4 Q. What about for water column samples that 5 are not reflected in a published paper or otherwise 6 covered by one of these Department of Environmental 7 Protection plans? 8 A. Then you would have to ask the individual 9 who collected the samples what mechanism they used. 10 I mean, of those mechanisms I would say they 11 basically only used one at the time. So it's not 12 that difficult. 13 Q. I would like to ask you the same question 14 about the soil sampling methodologies. How would one 15 know from examining your records what methodology was 16 employed for a given sample set? 17 A. Most everything is published under that 18 circumstance. The other would be, again, just by 19 asking the individual who collected the samples, 20 which is myself for the most part, and then for other 21 projects, again, those that are requiring either a 22 DEP or EPA project plan and description. Those 23 projects have been described under those 24 circumstances. 25 Q. You indicated regarding the water column 867 1 sampling that the predominant way of doing it was the 2 sample bottle with the protocol for it. Was there a 3 predominant methodology utilized for the soil 4 sampling? 5 A. I would say for the soil sampling, no. We 6 pretty much -- it depends on the specific project 7 what the purposes are because there are very 8 oftentimes that we want to know what's in various 9 increments in depth in the core, and then we have to 10 use a coring thing. If we are only interested in, 11 let's say, the top ten centimeters then we would use 12 a grab sample in conjunction with cores. QC the data 13 or QA the data, whichever way you want to look at it. 14 You can't make that distinction as easily. 15 Q. In terms of the number of samples 16 collected, was one methodology employed more often 17 than the others? 18 A. It would be a real close tie between grab 19 samples and the PVC cores. It took us a long time to 20 convince ourselves that grab samples were as good as 21 the cores. 22 Q. Going back to your soil sampling methods, 23 in the canals, was that done pursuant to a particular 24 protocol or QA/QC plan? 25 A. The EPA actually conducted that sampling. 868 1 I was along on several of the trips. They had with 2 my help designed a protocol plan. I have reviewed 3 the plan. 4 Q. When was this particular type of sampling 5 done? 6 A. The majority of it was done during the 50 7 canal stations that we examined last year some time. 8 Q. This is basically part of the mercury 9 measurements? 10 A. Mercury phosphorous measurements in the 11 sediments of the canals. 12 I have a clamshell box full myself. I 13 can't say that I have not used it. We found it to be 14 very difficult to use in the marsh, so we don't use 15 that. 16 Q. Now, the eggshell core device, was that 17 sampling done pursuant to an established protocol or 18 QA/QC plan? 19 A. The device is probably something I wasted 20 $900 on. It was a sampling device. We went out and 21 found out a $2 piece of PVC pipe worked just as well, 22 if that answers your question. 23 Q. Are you saying you really don't use it? 24 A. If you want to buy it, you can have it. 25 I'll give you a deal. 869 1 Q. Now, concerning the PVC cores, was that 2 done pursuant to an approved protocol or QA/QC plan? 3 A. It's a pretty standard method. It's 4 basically taking a sharpened piece of PVC pipe and 5 putting it through the soil. It doesn't require a 6 lot of training. 7 We have now incorporated that into our 8 comprehensive QA plan. That's in the process of 9 being submitted right now. But there is nothing 10 really particular or awe inspiring in that method. 11 Q. What about the grab samples? 12 A. Those are described the same type of thing. 13 We are putting that into our QA plan at the time. 14 Mostly described in the publications, though. 15 Q. This is not part of an approved EPA or DEP 16 protocol or QA/QC plan? 17 MS. PONZOLI: Object to the form. 18 THE WITNESS: As far as I know, the EPA has 19 no restrictions on how you collect the samples. 20 It's an analysis. And as long as you do sample 21 collection in the way it doesn't contaminate the 22 samples, there is no -- to my knowledge, any 23 kind of a written standard methodology for 24 collecting soil samples. 25 BY MR. HYDE: 870 1 Q. Now, as to the coring device for, I guess, 2 establishment of soil profiles, was that done pursuant 3 to some protocol or QA/QC plan? 4 A. All of these things are in project specific 5 plans where you describe what you are going to do. 6 People look to see whether they are contaminated. 7 With any of these methods, none of them are given 8 EPA's approval or sanction or DEP as being this is 9 how we wanted you to do it. If you don't do it this 10 way, you have to describe them and show how you are 11 going to keep cross contamination from coming out of 12 samples and those types of things. 13 But it's a project specific description. 14 It's not like a chemical analysis where you need to 15 utilize approved techniques if you want to have a 16 certification. 17 Q. That's why I used the more general term 18 protocol. It could be one that it self establishes 19 as opposed to someone else, would that be correct? 20 A. I would hesitate to agree with that. 21 Q. Does this protocol have some official title 22 to it? 23 A. There are approved protocol and there are 24 disapproved protocols and there are experimental 25 protocols, a number of things that have -- especially 871 1 if you are in the process of doing water or waste 2 water or sewage or something that needs to be 3 certified by HRS or another organization. 4 Q. Which of the soil sampling methodologies -- 5 you identified five of them -- fall within the 6 category of approved methodologies? 7 MS. PONZOLI: Object to the form. I think 8 you asked and answered that. 9 THE WITNESS: I indicated basically there 10 are no -- for soil sampling, there are as many 11 ways as you can -- I have listed five there. 12 And there are maybe 50 or more. I don't know. 13 There are many, many, many, ways to collect 14 a sample. That is not what is examined under 15 approval or disapproval under the circumstances. 16 BY MR. HYDE: 17 Q. I think I have a pretty good handle on how 18 a sample bottle is utilized for a water column 19 sample. 20 Can you describe for me the syringe 21 approach that you noted earlier. How does that -- 22 how was that done? 23 A. The syringe approach is very often in the 24 later stages of the marsh drying down. The water is 25 very shallow so you want to somehow be able to sample 872 1 that shallow water. It's of interest to what's 2 happening in that pool, more of a scientific aspect 3 of any of the nutrient cycles. 4 You go to the very shallow appeal because 5 of the syringe -- we are talking about a syringe made 6 for an injection -- you would be really sick if you 7 used these. These are 120 mil syringes. But they 8 are about an inch and a half in diameter, but they 9 have the same lower fitting on the base of them that 10 allows you to place the syringe in very, very shallow 11 area of water. 12 You simply draw up a sample into the 13 syringe, rinse the syringe three times by shaking and 14 squirting that water out so it's not contaminating 15 the bottle and collecting a final sample in the 16 syringe and placing it in the sample bottle. 17 Q. Once you transfer it to the sample bottle, 18 it's then treated in the same fashion as a sample 19 bottle would be? 20 A. In fact, the water is squirted into the 21 sample bottle. It's rinsed in the same manner. If 22 you were collecting the sample under water, rinsing 23 the bottle, everything is the same. There is one 24 more step involved collecting the water first in a 25 syringe because you can't submerge a sample bottle in 873 1 a shallow body of water. 2 Q. Do you reuse the syringes? 3 A. The syringes are reused. They are rinsed 4 at every location and they are rinsed with distilled 5 water and light free water, if you want to call it 6 that. It's not a very common mechanism for sampling 7 in the marsh. I'm not sure how many times an 8 individual syringe would be used. We take a number 9 of them out with us. 10 Q. Describe for me, if you will, the pumping 11 or vacuum device that you alluded to earlier. 12 A. Sure. Again, in places where you can stir 13 up the sediment or the water column is fairly 14 stagnant, you want to get an idea of what is in 15 there, we have a device that sits a fixed distance 16 off the bottom, approximately five centimeters off 17 the bottom, has holes in it and a piece of plastic 18 tubing going to a vacuum flask. 19 You place the device on the area. You let 20 it sit there until all the sediment settles, until 21 the existing conditions re-establish themselves, and 22 then you simply hook this vacuum pump up to the 23 vacuum flask and the tube from the sampling device 24 and draw the sample into the flask and discard that 25 and rinse it and then collect the sample that you are 874 1 going to analyze. 2 Q. Once you have done that, is it also 3 transferred to a typical sample bottle and then 4 treated? 5 A. Yes. 6 Q. So the methodology from that point on is 7 the same as you would employ for a typical sample 8 bottle? 9 A. The collection techniques are the only 10 things that vary. 11 Q. Would that be the same for the pail 12 methodology? 13 A. Yes. The pail methodology again in some 14 places it's -- it would be life threatening to try to 15 get down to the edge of the water or do something 16 like that. So we have to come up with a mechanism 17 for doing that for checking the surface sample. So a 18 clean pail is a way of doing it. Actually this is 19 one of the approved methods, if you will, 20 surprisingly enough. 21 Q. That sample is transferred to a sample 22 bottle and then treated the same? 23 A. That's correct. 24 Q. Describe for me if you will the Niskin 25 bottle method. 875 1 A. Okay. A Niskin bottle is a -- was invented 2 I guess probably in the early 1950's, maybe earlier 3 than that. I think it was in the 1950's. It's 4 designed primarily for oceanographic sampling. There 5 were various numbers of designs of Niskin bottles. 6 The one I have is one where there is no internal 7 parts, if you will. The older designed bottles had 8 rubber or metal springs on the inside that would pull 9 two caps over a piece of -- what a Niskin bottle is 10 is PVC with two caps on either end. They are opened 11 up. You send a messenger down the line which is 12 nothing more than a brass weight. It hits a 13 triggering device and causes the bottle to close. 14 The mechanisms of closing is what is 15 undergoing transformations for the last 37 or so 16 years. I happened to have the latest version of 17 this. We did the trials on it. It has an external 18 sampling thing and the bottles are also teflon lined. 19 Q. What utility does a Niskin bottle sampling 20 methodology serve in the Everglades Protection Area? 21 A. People are using Niskin bottles and I think 22 they are mistaken for doing that. It's not a very 23 good sample device for the marshes. They were 24 designed for oceanographic purposes. We bought it to 25 sample off of the same places where we are using the 876 1 budget. In fact, that's what we did. 2 We compared the Niskin bottle to the bucket 3 sample. Once we convinced ourselves the bucket was 4 as good as the Niskin bottle, we went strictly to 5 using a bucket sample for that. They call them 6 bailers, actually is the scientific name for it. We 7 used the Niskin bottle only when we are on hydro wire 8 on a ship. That's what they were designed for. They 9 weren't designed for collecting samples by hand. 10 They are used when you have -- depends. If 11 you wanted to collect a sample from the canal in the 12 bottom, then you have to use this kind of sampling 13 device. 14 Q. Once you have a water column sample in hand -- 15 by that I mean, it's in your sample bottle -- I would 16 like for you to describe for me not the methodology 17 but describe step by step how that sample was 18 transferred to your laboratory or a laboratory for 19 analysis. 20 Tell me if there is any different way 21 depending on how you sample that you might have 22 transferred a given sample. 23 MS. PONZOLI: Object to the form. 24 THE WITNESS: The bottles -- there are 25 multiple types of sample collected. There are 877 1 samples that are used for, let's say, turbidity 2 and microbiological parameters which are treated 3 in a different way than the samples which are 4 going to be looked at for dissolved nutrients. 5 BY MR. HYDE: 6 Q. I'm just concerned now about the samples 7 regarding total phosphorous and alkaline phosphatase. 8 A. Yes. Those are handled in the same way 9 turbidity and microbiological samplings would be 10 handled. Alkaline phosphatase is a biological 11 parameter. 12 It's important that those samplings are 13 analyzed as soon as possible. We have a procedure 14 that requires us to analyze those samples immediately 15 upon return from the field. So it could be as short 16 as an hour to as long as six to eight hours if the 17 samples are stored for those parameters. 18 We have found no difference in that time 19 frame by experimentation, but those samples are -- as 20 opposed to the other samplings, are not refrigerated. 21 They are kept at ambient temperature. If you 22 refrigerate them, you destroy the microbiological 23 parameters. 24 Q. Once you have the sample in the bottle, 25 what do you do with it? 878 1 A. It's placed in an ice chest. 2 Q. Nothing else in it? 3 A. No. It sits in an ambient temperature, 4 transported back to the laboratory and analyzed. 5 Q. That usually occurs between one and six to 6 eight hours from the time it was sampled in the 7 field? 8 A. That's correct. Total phosphorous 9 measurements do not have to be done immediately. You 10 can do total nitrogen because you are looking at 11 total nutrients. It doesn't matter what fraction 12 it's in, it's still analyzed in the fact that it's 13 total. 14 What we can do under those circumstances is 15 we generally refrigerate the samples after we have 16 taken the sample out for alkaline phosphatase given 17 it's late in the day. 18 If it's not late in the day, if it's 19 reasonable time, we actually process the total 20 phosphorous on the same day. We can generally 21 refrigerated those. Our recommended time is 24 hours 22 to have them prepared for analysis. 23 Q. What's the outer limit for testing them for 24 total nitrogen and total phosphorous? 25 A. I believe -- I have to look at our 879 1 comprehensive plan. I believe it's 24 hours we say 2 we do that. We do hold the samples until we are sure 3 that the analysis has -- in other words, if there is 4 a question about the data, we drop a vial or lose 5 something or whatever else like that, rather than 6 discard that, the sample is kept in the refrigerator 7 until it's analyzed, which could be as much as two 8 weeks. 9 But that is always noted on our data sheets 10 as to whether we had to do that. It doesn't happen 11 very often. But considering the total nutrients, 12 there is no indication that they change with time. I 13 think EPA protocol says from 28 days. That's if I'm 14 not mistaken. 15 Q. Concerning the initial phase of one to six 16 or eight hours, are you referring there to alkaline 17 phosphatase? 18 A. Any other microbial parameter. But under 19 your question, alkaline phosphatase. 20 MS. PONZOLI: Can we break at this point? 21 MR. HYDE: Yes, this is a good breaking 22 point. Afterwards we will get to the soil 23 samples from the field to the laboratory. 880 1 (Thereupon, a brief recess was taken, 2 after which the following proceedings 3 were had:) 4 BY MR. HYDE: 5 Q. Dr. Jones, I would like to ask you some 6 questions about how your soil samples which were 7 taken from the field to the laboratory. 8 First of all, once you had all of the 9 samples in hand from whatever sampling methodology 10 you employed, did you treat them the same way? 11 A. Yes. 12 Q. How was that? 13 A. Samples were in the field. Large roots, 14 snails, rocks, whatever non-representative material 15 or material that could bias the sample one way or the 16 other was picked out using pretty much standard 17 protocol. Roots that are living are white. You take 18 those out and throw them out. That sample is then 19 placed into a polypropylene sample cup, labeled and 20 then generally placed in an ice chest, cooler and 21 transported back to the laboratory. 22 Q. Are those samplings refrigerated or 23 otherwise cooled? 24 A. Most of the analysis we have done are total 25 analysis. 881 1 Q. By total -- 2 A. Total nutrient phosphorous and nitrogen. 3 In fact, all of the analyses are microbial. So no, 4 you will not refrigerate them. 5 Q. I think you stated that foreign matter such 6 as rocks and live roots were taken out pursuant to 7 some standard protocol; is that correct? 8 A. That's correct. 9 Q. What is that protocol? 10 A. Basically when soil scientists long ago 11 recognized if you want to find out what the soil 12 fertility is or if you want to look at soil nutrient, 13 you don't want to go next to the corn plant and get 14 all the corn roots in your sample. 15 What you are looking at is the matrix of 16 soil, not the matrix of plant roots. If you want to 17 analyze plants roots, you analyze plant roots. The 18 protocol states you have to remove that type of 19 material because it's not representative of what you 20 are looking for. 21 There are cases where the soil down here is 22 really limestone. Under that circumstance, you don't 23 go out and removal the limestone, so I don't want you 24 to get that idea. But in the area of, say, for 25 instance, the marsh or whatever, generally rocks are 882 1 not in there. 2 But sampling like we did in the Everglades 3 agricultural area, considering those are fields, very 4 often that would be a large chunk, a piece of rock or 5 limestone in the sample, and that's removed because 6 it's not soil. 7 Q. You specifically mentioned removing live 8 roots. Does that imply that you leave dead roots in? 9 A. That's correct. Dead roots are 10 representative of the soil. That's part of the 11 genesis and decomposition of the soil, is dead plant 12 material. That's what it's made up of. Peat is by 13 definition partially decomposed plant material, and 14 dead roots would be a portion of that. 15 Q. You use a sieve or a grate or some other 16 device to separate out this foreign matter or do you 17 just do it by hand? 18 A. It's done by hand. 19 Q. So one could still get maybe small rocks or 20 small live roots or something like that? 21 A. Yes, material that goes on -- generally 22 there are other steps. This material is dried in the 23 laboratory so there are other screening techniques 24 that come into play when it's dried and ground. And 25 not all materials grind the same way. 883 1 Q. So then you take it and put it in a ice 2 chest and you take it back to your lab? 3 A. That's correct. 4 Q. What do you do once you get it back to the 5 lab? 6 A. Depending on what analyses are going to be 7 taking place, if it's a mercury sample it will be 8 frozen at minus 20 degrees centigrade. If mercury is 9 going to be analyzed, it doesn't matter what others 10 are going to be analyzed. We freeze the sample after 11 taking out samples that we are going to use for 12 microbial or biological activities. 13 If it's just going to be analyzed for total 14 nutrients, say total phosphorous -- which is the 15 predominant -- or total nitrogen or total carbon, the 16 cap is removed from the sample bottle, placed in a 17 drying oven and air dried for 12 hours. 18 Q. Is this dryer a particular device or you 19 are saying it's just a common type of kitchen oven? 20 A. No, scientific device. Even though it may 21 look like your typical Kenmore, it's priced at such 22 and they will never admit that they take the tag off 23 and raise the price by about 300%. It's nothing more 24 than a forced air oven. 25 Q. Like a convection oven? 884 1 A. Well, it's a convection oven with a blower 2 on it. So there is forced air coming up through 3 this. It's not just convection. 4 Q. How long does it dry? 5 A. It's dried for 12 to 24 hours. Generally 6 12 hours overnight. 7 Q. Why is there such a spread? 8 A. It doesn't matter. It's dried to where 9 there is no water activity. It doesn't make any 10 difference. You could dry it for six weeks, but that 11 would take your oven space up. You don't want to 12 remove it too soon because then it will still have 13 water. 14 The way you determine that is you take your 15 particular soil, place it in an oven, weigh it -- I 16 should say weigh it before you place it in the oven. 17 You keep weighing it until it's no longer losing any 18 weight. That's how you determine the length of time 19 that's necessary. 20 Q. At what temperature does this drying 21 process take place? 22 A. 80 degrees centigrade. 23 Q. Is that temperature maintained uniformly 24 throughout the drying period? 25 A. Yes. 885 1 Q. Once you finish drying the soil sample, 2 drying the soil sample, what do you do? 3 A. The sample is milled in a Wiley mill. 4 Q. What do you mean by milled? 5 A. Ground. 6 Q. What is a Wiley mill? 7 A. W-i-l-e-y mill. Any soil scientist -- I 8 mean I'm unfamiliar with any others. It's a standard 9 mill used for grinding soil samples, dried samples 10 for science. 11 Q. So it's milled in the Wiley mill. What 12 then do you do with it? 13 A. The sample is collected and placed in a 14 glass scintillation vial for storage. 15 Q. Once you have the vial in storage, then you 16 want to analyze it for, say, total phosphorous. What 17 do you do with it at that point? 18 A. The sample would be taken out, weighed to 19 the appropriate amount that you are using for that 20 particular analysis. I believe total phosphorous is 21 25 milligrams. You weigh out 25 milligrams into 22 three additional vials because we do the analysis in 23 triplicate. Then we add some magnesium sulfate in 24 sulphuric acid and we oxidize the organic carbon away 25 and convert all of the phosphorous organic forms of 886 1 phosphorous into orthophosphates by a technique 2 called ashing. 3 Then we hydrolyze the ash and measure 4 orthophosphate on an auto analyzer. 5 Q. Is orthophosphates the same thing as saying 6 total phosphorous? 7 A. At this point it is because what the 8 purpose of the ashing is to converts all of the 9 phosphorous forms into orthophosphorous. 10 Q. Is this process done pursuant to some 11 standardized methodology? 12 A. Yes, it is. 13 Q. What is that methodology? 14 A. There is the preparation process, which is 15 different than the analytical process. There are 16 multiple procedures that are used. 17 Q. Take me through them step by step. 18 A. There is ASTM -- and I'm sorry, right now I 19 could probably guess at what it is, something 20 standards in testing materials. It's the code book 21 for different techniques, different ways to analyze 22 these things. ASTM method for ashing. 23 There is also a soils standard method for 24 ashing, and those two techniques combine for the 25 preparation. 887 1 Q. Is that also an ASTM method? 2 A. All of these methods cross reference each 3 other. You will have one manual written for drinking 4 water, another manual written for soils. You have a 5 manual written for sludge. 6 You have some standard -- just reference 7 materials that are around. They all tend to cross 8 reference each other. There are EPA methods. There 9 are AWWA methods. There are a number of different 10 publications that you can reference. So I sort of 11 hesitate to answer with a simple yes to that 12 question. 13 Q. Well, do you not use one particular 14 reference point for doing this? 15 A. They all are essentially the same. It's 16 just that one says this is how you do it for sludge 17 and the other one says this is how you do it for 18 sediment and the other one says this is how you do it 19 for soils. So it's sort of pick and choose. 20 I'm just pointing out that the technique is 21 the same for all of those, it just depends which 22 manual you particularly use, but they all cross 23 reference each other. 24 Any one of them are approved and we have 25 those all listed down and we are saying it's in our 888 1 comprehensive plan. They are all listed as methods 2 of use. 3 The method for analysis of the phosphorous 4 is simply we reference back to the EPA method. 5 Q. Which EPA method is that? 6 A. I have to look at the table. But it's the 7 ascorbic acid method for measurement of phosphorous. 8 Q. Are those all of the standardized methods 9 that are utilized when analyzing a soil sample for 10 phosphorous? 11 A. Yes. We don't do anything particularly 12 unique in the methodology portion of the thing. I 13 mean, there are other -- we have our instruments 14 standardized at lower levels. We optimize our 15 strengths for determining lower levels. 16 But that's much more prevalent for the 17 water column work than it is for the soils. The 18 soils are pretty much there. 19 Q. Once you reduced it to your 25 milligrams 20 sample, for lack of a better term, what do you do at 21 that point? 22 A. That's the sample that's added and then 23 hydrolyzed, meaning putting it back in the water. 24 And then the water is placed onto the auto analyzer. 25 Q. And what is the auto analyzer? 889 1 A. The auto analyzer is an automatic or a 2 continuous sampler and an analysis that then 3 automatically reports the values for phosphorous 4 contained in the sample. 5 Q. Is that a particular machine or 6 manufacturer's product? 7 A. Yes, it is. 8 The instrument that the company would have -- 9 I called it an auto analyzer, that's a rendered name. 10 I actually have RFA 300, which is 11 manufactured by a competing company. But it's a 12 standard device. DEP has them, DERM has them, South 13 Florida Water Management District has them. 14 They are a common instrument? 15 Q. Once provide the sample to the auto 16 analyzer, does the machine then in effect take over 17 and do the remainder of the work? 18 A. As much as a machine can. I mean, there 19 are sometimes a number of operations that have to be 20 manually put into the machine. The data has to be 21 checked, QA, upon the end of the analysis to 22 determine whether it needs to be reanalyzed or that 23 type of thing. 24 Q. But does the machine then, I guess, print 25 out, for lack of a better term, the results of the 890 1 analysis? 2 A. That's correct. 3 Q. And how is this printout done? Is it, for 4 example, just a raw data that you are referring to 5 earlier as being the standard computer sheet 6 printout? 7 A. That's correct. 8 Q. Once you have the raw data, what do you do 9 with it? 10 A. QA it. 11 Q. What does that QA process entail? 12 A. The first thing would be to examine all of 13 the output stored on a hard disk for a short period 14 of time. 15 And so the first thing we do is in the 16 process of examining what each one of the individual 17 peaks look like, see if there was an air bubble to 18 make certain that the computer program discriminated 19 the peaks in the right manner. So there is a visual 20 observation, a check that is done at that point. 21 The data then is printed out in hard copy. 22 We then make comparisons of the duplicate cups. So 23 if you have -- we generally end up with six cups. 24 Two duplicates of three samples or perhaps just two 25 duplicates of two samples. It depends what analysis 891 1 and what kind of statistics we want to run on it. 2 But there is a minimum of four sample cups 3 to look at. So what you would be doing would be 4 looking at that set of four numbers. And if one 5 number is plus or minus two standard deviation units 6 from the mean, that cup can be discarded as an error 7 associated with that particular sample. 8 These are generally very obvious. I mean, 9 there was air bubble that passed through the cell, 10 it's usually not two or three times the standard 11 deviation. It's on the order of magnitude 12 difference. That's the initial review. 13 The numbers are then averaged together to 14 produce a final result for that particular soil 15 sample. 16 Q. Then once you have the averages, what do 17 you do with them? 18 A. They are recorded in a data sheet. If it's 19 for our purposes, we will save it until we have 20 enough to make a publication, a scientific 21 publication, or we will turn it over to the various 22 groups of people who have requested the data. 23 MR. HYDE: Anyone have an objection to 24 taking a break for lunch now? 25 MS. PONZOLI: No, that's fine. Let's be 892 1 back at five to one. 2 MR. HYDE: An hour from now. 3 (Thereupon, a lunch recess was taken, 4 after which the following proceedings 5 were had:) 6 MR. SAMS: Dr. Jones my name is Gary Sams. 7 And by consent of Bill Hyde, who has been taking 8 your deposition, I'm going to ask you some 9 questions about documents and a few additional 10 questions. 11 Upon the conclusion of my questions I will 12 turn the matter back over to Bill to carry on. 13 Anything else you need? 14 MS. PONZOLI: I guess you would need my 15 consent also, but that's probably a small point. 16 MR. SAMS: I assume that we had your 17 consent, but you can give it if you wish. 18 MS. PONZOLI: Yes. 19 CROSS EXAMINATION 20 BY MR. SAMS: 21 Q. Dr. Jones, I'm going to hand you a document 22 that the United States furnished us following your 23 last deposition. 24 What I would like to do primarily with this 25 document -- 893 1 MS. PONZOLI: This is not in the pile we 2 supplied this morning. 3 MR. SAMS: This was faxed to me after the 4 last deposition. I will need one copy to ask 5 questions about. 6 MS. PONZOLI: He will need one to look at. 7 Does he have one? 8 MR. SAMS: Yes, he does. 9 MS. PONZOLI: I'm looking with him. 10 MR. SAMS: If you don't mind, I would just 11 like to have you go through these -- let's mark 12 this as Exhibit 89. 13 (The documents referred to were thereupon 14 marked Composite Exhibit 89 for Identification.) 15 BY MR. SAMS: 16 Q. This is a composite exhibit. I would like 17 to just go through this and have you identify to the 18 best of your ability what these things are. 19 I think we discussed some of these at your 20 prior deposition. I don't intend to dwell on those. 21 However, if you would indicate that we did examine 22 you about those at the prior deposition it would help 23 me. 24 Page No. 3, I'm referring to the fax pages 25 at the top right-hand corner. Is that the latitude 894 1 and longitude designations for the E-map 50 canal 2 station survey? 3 A. Yes, it is. 4 Q. Could you identify for me in short form for 5 the record pages 4 and 5? 6 MS. PONZOLI: I don't know if I understand 7 the question. Do you understand the question 8 Dr. Jones? 9 THE WITNESS: Yes. These are, as it says 10 at the top, Everglades canal fish samples which 11 would be Gambusia, mosquito fish. And the 12 numbers designated in the thing, 31 A, B, C, D 13 and E those represent five individual fish 14 collected at site 31 as indicated on page 3. 15 And then there would be their mercury 16 concentrations in the next column. 17 BY MR. SAMS: 18 Q. There are three columns of data or three 19 sets of three columns of data. I believe we saw this 20 at your last deposition? 21 A. I don't know if this is complete. I was 22 not counting up all of the numbers on here. There 23 are additional sheets that have this information. I 24 think this is all of it. 25 MR. HYDE: Can I ask just for clarifying 895 1 the record, what is the measurement of 2 concentration of -- the mercury concentration in 3 the individual fishes? 4 THE WITNESS: Listed under HG concentration 5 parts per billion or nanogram per gram. 6 BY MR. SAMS: 7 Q. Are you familiar with the health advisory 8 limits of the state government? 9 A. Yes. 10 Q. And what units are those expressed in? 11 A. Parts per million. 12 Q. Dr. Jones, is the fax page 6 the same data 13 tabulated differently for the fish? 14 A. I believe it's the average of them, 15 although there are places where I believe some of 16 the -- no. There are some missing points in here, 17 and those were -- have been presented in a later set 18 of documents. 19 You may have those either today -- or I may 20 be mistaken. Those may have been bottom samples and 21 there was no fish taken on those. The B in the first 22 column and the station would indicate bottom sample. 23 So that means there were no fish with the blanks. 24 This is complete average data. 25 Q. Where the phrase no fish appears, does that 896 1 mean that the data are to be found elsewhere? 2 A. No, it means there were no fish collected 3 at that site because there were no fish to be 4 collected at that site. Some of these were anoxic 5 waters. 6 Q. Fax page 7, can you identify what this? 7 And perhaps it continues on to pages 8 and 9. 8 A. That's correct. Pages 7, 8 and 9 are 9 actually one sheet of a computer printout, one long 10 sheet. And these are data of mosquito fish collected 11 in the EAA during our entry and the mercury 12 concentrations. 13 Q. You say these are in the EAA? 14 A. Yes. 15 Q. What code or other identification would 16 enable us to identify the site from which these fish 17 samples were collected? 18 A. If you see in the sample ID, we can go to 19 the first page where it says sample ID, and it says 20 standard 1 through 8. Then it has blank, blank and 21 then 9-1, 9-2 and 9-3. 22 Those would be the individuals collected 23 from that particular -- from site 9 in that survey 24 and then 10-1, 11, 12. Those would be all of that. 25 You had been provided all of those which would tell 897 1 you where those would be located in the EAA. 2 Q. That's the map that we examined before 3 during your deposition? 4 A. Yes, along with my field notes which you 5 had me read into the record -- or someone had me read 6 into the record. 7 Q. What does the third column of the data 8 represent, imported PH? 9 A. It stands for peak height. This program 10 wasn't run by the same machine that was doing the 11 analysis. So we took those peak height values and 12 imported them into a Lotus program. We were able to 13 process the data while the instrument was running 14 other samples. 15 Q. So that was, in essence, a mechanical entry 16 that doesn't affect the ultimate? 17 A. Truly not mechanical. It means the data 18 goes from the instrument, analytical mercury 19 detector, onto a disk. That disk, instead of 20 being -- instead of using the Lotus program on that 21 computer, we take that disk and put it on another 22 computer and import that file into a Lotus spread 23 sheet on there. 24 It's just a way of making -- being able to 25 do mercury analysis at the same time you are doing 898 1 the data crunching or the number crunching. 2 Q. Does that column of numbers get used in any 3 way in interpreting the balance of the numbers shown 4 on the chart? 5 A. Yes. They are the same numbers we could 6 have called it peak height, imported. It means we 7 took it from one computer and did it on another one. 8 That probably doesn't need to be there, the word 9 "imported." 10 Q. Recognizing you are talking to somebody who 11 is pretty illiterate in computers and scientific 12 field tests. What's a peak height, again? 13 A. A peak height is when the instrument is 14 measuring a sample -- in this case, mercury. The 15 higher the concentration of mercury, the higher the 16 little line loop, the trace. So it goes up to a 17 certain level at the highest level. That's the peak 18 height, it is that point and it drops off at the 19 sample, goes out of the carrier stream. 20 Q. I assume my experts will be able to figure 21 out what that means. 22 Which column ultimately was the number that 23 you resolved as the concentration of mercury and 24 tissue? 25 A. It would say correct mean HG, nanogram per 899 1 gram. 2 Q. The next-to-the-last column? 3 A. That is correct, right before the standard 4 deviation. There are too many decimal places, but 5 other than that. 6 Q. I take it that the reference numbers have 7 nothing to do with the 50 canals surveyed; is that 8 correct? 9 A. No, they don't. They had something to do 10 with -- it's a column that's placed in. It also had 11 something to do with the label of the samples for 12 making sure the right weights go to the right place 13 and all of that kind of stuff. 14 Q. On pages 10, 11, 12, are those similar runs 15 for mercury concentrations in fish tissue taken in 16 the Shark Valley of the Park or and the Park? 17 A. They were taken in the Park in Shark 18 Valley. 19 Q. And when were these samples taken? 20 A. That's a good question. Probably in the 21 December, January time frame. 22 Q. Of '92 or '93? 23 A. Of '93 and '94. 24 Q. How does one identify the location from 25 which these samples were obtained? 900 1 A. They were all collected -- they were all 2 collected at a site out near the center of the slough 3 down by the tower. This is just a series of numbers. 4 We were looking at size ratios, sex determinations 5 and stuff like that. 6 This is preliminary information. I 7 don't -- other than putting it on a map, that's the 8 only thing I would be able to tell you about this. 9 Q. Why don't we consider doing that. I'm not 10 sure this map is very satisfactory. It's a well worn 11 map. Why don't we mark this as the next exhibit. 12 MS. PONZOLI: I don't mind if you mark it 13 as an exhibit. I won't have Dr. Jones drawing 14 on the map, but he can tell you it's in the tram 15 trail. And you can identify the tram trail 16 which is probably visible on the map. 17 THE WITNESS: It's not. That's the problem 18 with the map, none of the landmarks are 19 featured. I can point to the general area. I'm 20 not going to be real close because of the fact 21 that the landmarks aren't on this particular 22 map. 23 MR. SAMS: Let's do the best that we can. 24 We will mark it as Exhibit 90. 901 1 (The document referred to was thereupon 2 marked Exhibit 90 for Identification.) 3 MS. PONZOLI: The tram trails exist on 4 other maps fairly defined, it's not an unknown 5 landmark for the Park. 6 BY MR. SAMS: 7 Q. Could you point to the approximate 8 location? 9 A. It would be approximately right there. 10 Q. Are you pointing to the west of a label 11 that says L-67, and roughly to the south of the label 12 that says L-29? 13 MS. PONZOLI: I don't mind his telling you 14 approximately in words, but when you put the dot 15 on the page or he puts it, it becomes almost the 16 same at some point. 17 So you are welcome to mark your map however 18 you wish. I will only have my witness give you 19 a verbal representation. 20 MR. SAMS: That's all I asked him for. 21 MS. PONZOLI: I see your pen poised to 22 mark? I want to make sure -- 23 BY MR. SAMS: 24 Q. Can you tell me? 25 A. That's approximately correct. I feel much 902 1 more comfortable if it were a different map. Again, 2 this data is just very preliminary for that purpose. 3 Q. Is that point somewhat to the west of and 4 above the first D in Dade, again recognizing you are 5 describing only an approximation? 6 A. Yes. 7 Q. Now, I take it these were analyzed in a 8 similar manner to the ones we looked at, pages 7 9 through 9; is that correct? 10 A. That's correct, although there were some 11 fish in here that had large enough size to them that 12 they had to be split in half. Again, this is an 13 unusual data set. 14 Q. Why does that make it unusual? 15 A. This is the data set that we -- again, this 16 is an experimental -- it's a set of data we had no 17 intention of doing anything with other than going out 18 and getting an idea what mercury concentrations we 19 are looking at. 20 Are we going to see high mercury 21 concentrations, low, what size fish can we throw in 22 the vile without having it explode in the autoclave? 23 It wasn't a data set to be used for any type of -- we 24 are not going to use this data set for making any 25 interpretation or making any comments on any mercury 903 1 anywhere in the Everglades. 2 Q. Was there a problem in the analysis? 3 A. No. There is no problem at all. It's just 4 if we don't utilize data, that we don't have a 5 specific purpose. It helps me form background 6 knowledge of the system and analytical techniques 7 that are going to be necessary. 8 But it's not a set of data that's going to 9 be used for making any kind of publication or 10 anything like that. It's just preliminary 11 information which will go on defined experimental 12 protocol sampling designed to do this data properly 13 when we have that goal in mind. 14 Q. And these data were all from the same site; 15 is that correct? 16 A. They were all from the same location. 17 Q. Was that in the marsh? 18 A. It was in the marsh. 19 Q. Does that include the data on page 12? 20 A. Yes. 21 Q. What are those data on page 12? 22 A. The one with the number one besides them 23 are for Gambusia, and No. 2 is Poecillia. And I 24 don't have the faintest idea what that is. 25 Q. I see the word "oyster." 904 1 A. That's our standard reference material from 2 the NITS reference material that we run to check and 3 see how well our analyses are turning out. 4 Q. Do any of these columns display calculated 5 mercury concentrations in tissue? I'm referring 6 again to page 12? 7 A. I don't believe so. I think this is a data 8 set that has gone no further. The first thing would 9 be peak height. It's possible that the second column 10 is an uncorrected mercury concentration, but I don't 11 believe it is. 12 Q. Then I would direct your attention to pages 13 13 and 14 and ask you where these data were 14 collected. 15 A. It says at the top, crayfish research road 16 Everglades National Park. It would be to the best of 17 my knowledge the road heading towards the research 18 center alongside the road there to collect some 19 crayfish. 20 Q. In a canal? 21 A. I presume so. This is a portion of Bill 22 Loftus' data. And he was, again, just trying to get 23 a general idea of what kind of mercury concentration 24 to expect at various species, and this is what that 25 represents. 905 1 Q. I see in pages 15, 16 and perhaps 17 2 another data set. Do those go together, those pages? 3 A. Yes, they do. 4 Q. Where were these data collected -- or these 5 fish, rather? 6 A. These would have been collected along the 7 tram road. This to the best of my recollection -- 8 these are again samples that were collected -- these 9 particular samples were collected by Bill Loftus, and 10 they were collected just right next to the air boat 11 launching ramp on the tram road. 12 Q. Is that near the other location? 13 A. It's approximately four or five kilometers 14 to the west of the other location, which is out in 15 the marsh. This is just next to the road. 16 Q. I'm sorry, you may have said in a canal or 17 in a marsh? 18 A. There is no canal there. So it would be 19 the marsh, as it is. 20 Q. Let me go back. I think you said the last 21 two sets of data we looked at were collected by 22 Dr. Loftus? 23 A. Mr. Loftus. He is working on his Ph.D. 24 Q. Did you collect any of the data or any of 25 the fish? 906 1 A. In the last two sets, no. 2 Q. In the first set, did you collect those 3 fish? 4 A. The ones that say EAA I collected. 5 Q. Did he collect all of the fish in the Park? 6 A. Yes. 7 Q. Were you with them at the time he collected 8 those fish? 9 A. No, I was not. 10 Q. How were they provided to you, in what 11 condition? Were they iced? 12 A. He did the analysis. They were iced. And 13 Bill collected them and analyzed them himself. 14 Q. At what laboratory? 15 A. At my laboratory. 16 Q. Is he someone whom you trained to use the 17 analytical equipment in your laboratory? 18 A. Yes. This is actually part of his 19 dissertation. This is the scoping portion of his 20 dissertation, to find out approximately what levels 21 he should be expecting in different levels of the 22 food chain. 23 Q. What is the next set that belongs together, 24 is it pages 18, 19 and 20? 25 A. That would be pages 18 through 23. 907 1 Q. Are those, again, fish collected by 2 Mr. Loftus? 3 A. They are various species. There are fish, 4 there are invertebrates. I know one of these 5 organisms is a spider. Just about anything we could 6 put into an ampule was digested and we attempted to 7 get some idea of what the limitations of our 8 ampulation technique is. 9 Q. Where is the mercury concentration in fish 10 tissue listed on these tables? 11 A. In this case it would be on pages 21, 22 12 and 23. If you turn to page 21, you would see it 13 listed in two columns there. The individuals are 14 listed in the second column from the left. It's 15 nanogram per gram and the means are listed in 16 nanogram per gram in the third column from the left 17 and the standard deviation in the last column. 18 Q. I take it the comment you made about not 19 intending to use these data in your work applies to 20 all of the ones obtained by Mr. Loftus; is that 21 correct? 22 MS. PONZOLI: Object to the form. 23 THE WITNESS: I don't know that I would 24 say -- I'm not intending to utilize these 25 numbers in any other manner than to just -- I 908 1 know now something about what spiders have in 2 the Everglades. They can't be chunked out of my 3 mind. I happen to know there is mercury in a 4 spider in the Everglades. That doesn't mean I 5 have any way of saying what every species of 6 spiders have, how statistically accurate that 7 is. 8 I know there is some mercury in spiders. 9 So, I mean, if there is that type of knowledge I 10 do intend to -- don't intend to throw it out. 11 BY MR. SAMS: 12 Q. In connection with this case you don't 13 intend to use this data? 14 A. I don't think so. I'm saying it's part of 15 my general background of mercury, so I wouldn't -- I 16 have no way of getting rid of that information, you 17 know. 18 Q. How would one identify which sample on 19 pages 18 through 23 is which critter -- which type of 20 critter? 21 A. If you look on page 23 there is a key. It 22 has 7, 6, 2 equal 5 equals on these tables. And it 23 says species and you see a list of 7. 24 Q. That's the code? 25 A. Yes. 6, 2, et cetera. 909 1 Q. I would like to ask you to identify some 2 other documents we have seen today. 3 I show you first what appears to be a 4 letter from Ron to Jerry dated March 1, 1994. Is 5 that from you to Jerry Tober of the EPA? 6 A. Yes, it is. 7 Q. What was the purpose of your writing that 8 letter? 9 A. We have been discussing for a long time in 10 the context of the remap proposal of things that 11 needed to be included that were more process oriented 12 rather than sort of field oriented measurements 13 mapping type of programs, since I'm more interested 14 in the processes involved in mercury and phosphorous. 15 He had requested that I send him a short 16 paragraph describing some of the things that I 17 thought were important in our considerations in sort 18 of process, transport process and of mercury so that 19 he could include this in the latest draft of -- I 20 believe it's the remap proposal. I'm not sure it was 21 a remap. It was on one of those drafts for the EPA. 22 Q. Have you revised this recommendation at all 23 since you made it to Dr. Tober? 24 A. I discussed it with him yesterday and it 25 hasn't been revised. He just thanked me for giving 910 1 it to him. And apparently he presented all of these 2 suggestions and they were agreed to. 3 Q. Agreed to by whom? 4 A. By whoever is in charge, I guess the 5 funding for them or whatever. To the best of my 6 understanding, there has been at least partial 7 funding of all of these particular things. I don't 8 know the level of the funding at this time. 9 MR. SAMS: I would like that marked as 10 Exhibit 91. 11 (The document referred to was thereupon 12 marked Exhibit 91 for Identification.) 13 BY MR. SAMS: 14 Q. The next document you provided us was 15 actually a set of documents clipped together. 16 Could you tell me first whether they belong 17 together and, if so, give me a description of what 18 each document is? 19 A. Okay. Starting with Bates No. 96353 and 20 ending with 89678? 21 Q. Yes. 22 MR. SAMS: I would like that marked as 23 Exhibit 92. 24 (The document referred to was thereupon 25 marked Exhibit 92 for Identification.) 911 1 MR. SAMS: Do you have an extra copy of 91? 2 I gave the reporter mine. 3 THE WITNESS: I have one. 4 BY MR. SAMS: 5 Q. If you could just answer the first 6 question, I guess to get it started. These belong 7 together. I think you said they did; is that 8 correct? 9 A. I haven't said anything yet. 10 Q. It appears now that I look at it these are 11 separate documents. 12 A. Without reading it in totality I would have 13 to agree that I think that is a separate document. I 14 don't have any reason to believe it's anywhere other 15 than it was in sequence in the file and it was just 16 clipped together. 17 MR. SAMS: Let the record reflect I'm going 18 to ask you to remove 9675 through 9678, if I 19 may. 20 BY MR. SAMS: 21 Q. With that removal, do they now address the 22 same subject? 23 A. Yes, they do. 24 Q. Could you briefly describe each of these -- 25 let me just ask you a series of questions, if that 912 1 may keep you from having to do a long narrative. 2 As I understand it, these documents relate 3 to a proposal that you and another investigator 4 proposed to do to establish a field dosing experiment 5 for development of the class 3 nutrient numerical 6 criterion; is that correct? 7 MS. PONZOLI: Object to the form. 8 THE WITNESS: Not really. 9 BY MR. SAMS: 10 Q. What do these relate to? 11 A. There was never a proposal made. There 12 was -- we were requested by the TOC to put together a 13 sort of scoping approach document type of a thing. 14 But I don't -- we called it a pre-proposal. There 15 has been a number of things in there. 16 I would hesitate to call it a full blown 17 proposal or anything like that. I mean, it was only 18 like three pages long or five pages at the most, I 19 guess. That's not quite what I would consider to be 20 a proposal. 21 Q. Do these all relate to that pre-proposal or 22 whatever the submittal would be? 23 A. It relates to that and the presentations 24 that were made to TOC and the District's staff on 25 this particular concept. 913 1 Q. By? 2 A. By myself. 3 Q. And then do the comments of other reviewers 4 comprise pages 9660 through 9663 and 9669 through 5 9670, and then 9671 through 9674? 6 A. Outside reviewers? 7 Q. Outside of the agency or the TOC. 8 MS. PONZOLI: I'm sorry, would you give me 9 the question again? You want to read it back? 10 MR. SAMS: I asked if those three documents 11 were comments by other reviewers. 12 MS. PONZOLI: On the pre-proposal? 13 THE WITNESS: Did you exclude the District 14 comments? 15 BY MR. SAMS: 16 Q. I did. But if that fits that category, 17 then I would amend it to include also 9664 through 18 9668. 19 A. Those are comments on -- most of these are 20 comments on a document that was sent to a number of 21 people as if it were a proposal, and there was never 22 a document prepared for such a review. But 23 nonetheless, these are comments upon that particular 24 document. 25 Q. Were these forwarded to you by the 914 1 District? 2 A. Yes, they were. 3 Q. The water management district? 4 A. I received them from the water management 5 district. I wouldn't say they forwarded them to me. 6 Q. How did you obtain them? 7 A. Some of them came at a meeting we had at 8 the District where they presented them and I picked 9 up a package of material which they contained. 10 Others were delivered to me by, I'll say, third 11 parties because I honestly don't remember who I 12 received them from. It could have been any one of a 13 number of people. 14 MR. SAMS: I'm going to ask the court 15 reporter to mark another document which is Bates 16 Nos. 9679 through 9688. Mark that as 17 Exhibit 93. 18 (The document referred to was thereupon 19 marked Exhibit 93 for Identification.) 20 BY MR. SAMS: 21 Q. I ask if that's a document -- do they 22 belong together? 23 MS. PONZOLI: Are you excluding 9685, 24 Mr. Sams? 25 MR. SAMS: Yes. 915 1 MS. PONZOLI: That's been removed? 2 MR. SAMS: Yes. 3 THE WITNESS: He is removing 85 from the 4 file. 5 BY MR. SAMS: 6 Q. Those were the pages that I asked you 7 about. Did those documents fit together? 8 A. Did you take 85 out? 9 Q. I'm sorry. I misunderstood your clarifying 10 question. I thought you said 7585 is part of this 11 document. 12 A. It's one document. 13 Q. What does this document consist of? And in 14 particular I'm interested, Dr. Jones, in the tables 15 1, 2, 3, 4 and 5. 16 A. This was something that was sent to me by 17 EPA on the 15th, I guess, of March, the date on the 18 bottom. It sort of gives me an idea how many samples 19 I would be handling in my laboratory for mercury and 20 various other types of analysis. 21 Q. Is that under the remap study? 22 A. I don't know whether it's remap or E-map or 23 I just considered it. EPA mercury study, I'm pretty 24 sure it's a remap, but it could change on a daily 25 basis. 916 1 Q. I show you what was stapled together and 2 includes Bates stamps 9686 through 9698 and ask you 3 if those documents belong together. 4 A. Yes, they do. 5 MR. SAMS: I'll have this marked as 6 Exhibit 94. 7 (The document referred to was thereupon 8 marked Exhibit 94 for Identification.) 9 BY MR. SAMS: 10 Q. Dr. Jones, have you reviewed the data that 11 are described in these papers that we have just 12 marked as Exhibit 94? 13 A. Yes, I have. 14 Q. If you could turn to page 9690, have you 15 formed any observations concerning those data? 16 A. The data is of such limited number, my 17 understanding this study was basically done as a 18 training exercise for the District's staff on how to 19 collect mercury samples. 20 It's my opinion that you really can't make 21 any interpretation from this data set. The only 22 thing I can say from it is that the values for the 23 concentration of both total mercury and methyl 24 mercury appear to be reasonable. 25 Q. Reasonable in comparison to what? 917 1 A. The data that we collected in the marshes 2 that -- the remap study, EPA. 3 Q. What data have you collected in the marshes 4 under the remap study? 5 A. We had a number of laboratory 6 intercalibration exercises. I think three with our 7 laboratory, Batel, Pacific Northwest Laboratory, the 8 Athens EPA laboratory and the Cincinnati laboratory, 9 to some extent. We have done work on that. 10 Then also in our development of the 11 speciation -- mercury speciation techniques we have 12 collected samples predominantly from the L-67 canal 13 and from water conservation areas 3A just to have 14 actual samples to work with. 15 These just appear to be in the same ranges 16 that we are finding in the same ranges that Batel is 17 finding. 18 Q. What number of fish have you analyzed for 19 mercury when the fish came from marshes under the 20 entire calibration exercise? 21 A. Fish were not included. 22 Q. Turning next to page -- Bates No. 9695, I 23 notice that the highest methyl mercury samples appear 24 to be from the 217 guage area in Water Conservation 25 Area 2A; is that correct? 918 1 A. In this table, yes, it does. 2 Q. Is that a phosphorous enriched or 3 phosphorous unenriched site, relatively speaking? 4 A. Relative to what? 5 Q. Relative to whatever you would consider to 6 be natural background, first. 7 A. I can't speak specifically for the 217 8 guage, but for sample station due south of S-10C at 9 approximately the same distance in as the 217 guage, 10 it would be an enriched area. 11 Q. And you don't know whether the 217 guage 12 area itself is enriched or unenriched? 13 A. I have never done any work at the 217 14 guage. It all comes in the definition what's 15 enriched and unenriched. I have different 16 parameters. 17 Q. When you say that the area that you spoke 18 of as being about the same distance from the 19 structure as the 217 guage, what phosphorous 20 concentrations of water were you referring to? 21 A. I'm not referring to it being enriched 22 because of phosphorous concentrations of water. 23 Q. On what basis did you refer to it as 24 enriched? 25 A. Decreased alkaline phosphatase activity. 919 1 Q. Do you know the phosphorous content of the 2 sediments at that location? 3 A. At the 217 guage? 4 Q. No. The alternate point that you stated 5 that you could identify the state of enrichment. 6 A. I do, but I can't name it without looking 7 at the table and seeing where the 217 guage is and 8 making a parallel and drawing a line through it to 9 see where it was. 10 Q. I think you have the set in the same order 11 that I do here. Can you tell me what the next page 12 is and whether it goes with any other page? 13 A. 9699 does not go with any other page. 14 MS. PONZOLI: Off the record. 15 (Discussion off the record.) 16 BY MR. SAMS: 17 Q. Can you tell me whether page 9700 relates 18 to any of the other pages it's with? 19 A. It's a single document. 20 Q. There is a reference to sampling the week 21 of the 11th. Is that April sampling, that's being 22 done? 23 A. My understanding is it has been put off for 24 a week but it will be happening somewhere around the 25 11th. 920 1 Q. What is the purpose of that sampling? 2 A. The U.S. Army Corps of Engineers has a 3 project to cut a gap between the L-67 A and C levees 4 to allow water to flow from Water Conservation Area 5 3A to Water Conservation Area 3B. This is some work 6 to see if there were any changes in vegetation 7 alkaline phosphatase or total phosphorous during the 8 course of that experiment, if you will. 9 Q. Who is Jim Stone? 10 A. He is a gentleman who works -- Doctor. 11 Dr. Jim Stone, he works in Plantation -- or 12 Fort Lauderdale, by the address. 13 Q. Can you tell me which of the next pages 14 belong together? It appears to be 9701 through maybe 15 9726. 16 A. That's correct. 17 Q. Have you reviewed this paper? 18 A. I have not. 19 Q. Was it provided to you by David Lane at 20 your request? 21 A. It was not. 22 Q. Do pages 9727 and 9728 relate to any of the 23 pages which follow? 24 A. Yes, they do. 25 Q. What pages belong together? Does it run 921 1 all the way through 9771? 2 A. Yes, it does. 3 Q. What is your purpose in obtaining this 4 manuscript? 5 A. This is a manuscript that Dr. Amador and I 6 put together. It was on Everglades soils and carbon 7 metabolism. This is just the latest version to be 8 submitted to Soil Science. 9 MR. HYDE: Can I see what the follow-up 10 page is? Mine has two letters. It says 11 Table 1. Okay. 12 BY MR. SAMS: 13 Q. Does this study have anything to do with 14 the effect of nutrients on mercury cycling in the 15 Everglades? 16 A. It doesn't have mercury cycling, no. 17 Q. Do pages 9774 through 9783 have anything to 18 do with the effect of nutrients on mercury cycling in 19 the Everglades? 20 A. No. 21 Q. Could you identify for me what pages 9784 22 through 9786 are? 23 A. These are total phosphorous in the water 24 column and non-purgeable from the water done in the 25 50 stations in the EPA study. 922 1 Q. Did these result in the phosphorous data 2 from -- that we have seen from the first round of 3 cycling -- first cycle of sampling in that study? 4 A. Yes. 5 Q. Are these data that you did not have at the 6 time of your last deposition? 7 A. I believe you have a copy of this. It was 8 presented in my documents. If it wasn't, I can't 9 imagine why it wouldn't have been. 10 MR. SAMS: Just to be sure, we've got to 11 mark this as Exhibit 95. 12 (The document referred to was thereupon 13 marked Exhibit 95 for Identification.) 14 BY MR. SAMS: 15 Q. Can you identify the documents that seems 16 to be stapled together and is comprised of page 9787 17 through 9789? 18 A. Yes. 19 Q. What is that? 20 A. This would be an example of the machine 21 output for total phosphorous analysis, and I believe 22 this is for EAA soils. 23 Q. This would have been taken on the EAA entry 24 back in March '93; is that correct? 25 A. I believe so, yes. 923 1 Q. And this is machine output from your lab? 2 A. Yes -- excuse me. This goes with another 3 set of documents in here. It may be mislabeled on 4 the top as EAA. It may need to be EPA. 5 At that point it would be sediments from 6 the canals. That's why I was looking through here 7 and picking out the other documents. 8 Q. Could you attempt to do that? 9 A. Yes. I don't believe that to be the case. 10 I just want to be certain. I believe it's mislabeled 11 and it should be EPA sediments, canal sediments. 12 Q. Is it possible to identify the locations 13 from which the samples were taken? 14 A. The number in pen and pencil written on the 15 side. 1, 2, 3, 4, 5, 6, 7, 8, 9. It should be 16 continuing on the next document, 9790 through 9794. 17 There should be 50 total stations. There also will 18 be some 205, 215, 235, those would be the duplicates 19 taken at stations 55, 15, and 35. 20 Q. So basically pages 9787 through 9794 are 21 related to each other in the manner that you 22 described? 23 A. Yes, to the best of my understanding, the 24 problem being that the label here, which I didn't put 25 on this table, this was my technician that wrote this 924 1 on, he has a tendency to get EPA. -- USEPA, 2 Everglades Protection Area and EAA messed up 3 sometimes. 4 Q. And these, to the best of your 5 recollection, will be Everglades Protection Area 6 sediment samples? 7 A. They would be EPA. Environmental 8 Protection Agency, the canal samples. I do that by 9 looking at -- if you would be willing, 9841 is the 10 summary table. 11 MS. PONZOLI: Do we need to remove that 12 from the document? That's inside. 13 MR. SAMS: I don't know where that is. 14 THE WITNESS: It would be in the last set 15 of documents. 16 MS. PONZOLI: Inside sequentially. 17 MR. SAMS: I may be overlooking something. 18 Mine seems to end with 9816, the set I was 19 handed. 20 MS. PONZOLI: Yours ends with what? 21 MR. SAMS: The last page I have is 9816. 22 MR. HYDE: No. 23 MR. SAMS: Off the record. 24 (Discussion off the record.) 25 BY MR. SAMS: 925 1 Q. You were about to explain to me, Dr. Jones, 2 something regarding the pages 9787 through 9794 3 utilizing the page with the Bates No. 9841? 4 A. That's correct. 5 Q. What were you about to tell me? 6 A. I compared sample No. 1 with the one on 7 9787, and you see that the average is 2302.66 under 8 the column labeled TP on 9841. 9 Q. Yes. 10 A. That appears to be the -- approximately the 11 average you would get by averaging those four numbers 12 together. So therefore, knowing that these are the 13 EPA canal sediments which I have labeled as such on 14 9841, I realize -- and since these documents were, 15 when they were handed to me, attached as one here, I 16 just happened to relabel the one top thing. 17 I had sent this to Dr. Tober at EPA. He 18 doesn't have any interest in having numerous pages of 19 machine output. So the summary table was sent and 20 that's why it was separated. 21 Q. Does 9841 necessarily go along with any 22 other page? For example, does it belong with 9842 23 through 9844? 24 A. I believe 9829 through 9844 was all in a 25 package of material that I sent to Jerry Tober at 926 1 EPA. 2 MR. SAMS: Why don't we to try to get the 3 record a little clearer. Mark as the next 4 Exhibit 9787 through 9794 -- Bill, in other 5 words, I'm leaving out for the time being 9841 6 so we can keep it in context. Mark that 7 Exhibit 96. 8 (The document referred to was thereupon 9 marked Exhibit 96 for Identification.) 10 MR. SAMS: Off the record. 11 (Discussion off the record.) 12 BY MR. SAMS: 13 Q. Describe for me document by document, 14 Dr. Jones, if you could, what the documents are that 15 start with 9795 and continue to the end. 16 If you could do that in a way that we can 17 identify pages that belong together, I would 18 appreciate it. 19 A. I'll indicate right now some of this 20 material has been produced simply because of the 21 request, you know, and that there will be some 22 duplicate pages or there will be pages that under 23 ordinary circumstances I would have discarded because 24 they are a later version of that that are more 25 complete. 927 1 Some of these are working documents where 2 Jennifer Tothos, who was the technician, would have 3 presented a table, left something out and said please 4 add this, you need to give me the means or no, that's 5 not the way we wanted the standard deviations. But 6 they are all of different variations on there. 7 Ordinarily I would have gotten those. 8 Q. If you could, identify each document and 9 who prepared it. 10 A. I'll try to do that, but I may not be able 11 to do that. I'll do it to the best of my ability on 12 the time frame like this, okay, because it would take 13 us longer. 14 The first document is EPA water samples 15 collected from South Florida Water Management 16 District from various structures within the Water 17 Conservation Area. 18 The table is not appropriately labeled. 19 There is no data on here. I'm not sure what the rest 20 of the values are. This is an early version of that 21 particular information. I would ordinarily have 22 discarded this table. 23 The second series of information started 24 with No. 9796 is results of analysis of fish 25 collected from the holeyland sites 1 and 7 -- not 928 1 holeyland sites, that wasn't received. It goes along 2 with the KBN data that was collected for Hopping, 3 Boyd, Green, I believe. These are just analyses of 4 replicate samples. The number that says these are 5 represented on the next pages with 9797 and going to 6 96798. You can see in there it has sample ID and has 7 HS-5 and 10-A. 8 Those have to do with the chain of custody 9 forms that we received from KBN indicating where the 10 fish sample was located. 11 Q. Which numbers identify station numbers, 12 sampling station numbers? 13 A. It would be in the sample. You have to go 14 back to the data sheets, chain and custody forms 15 which had been produced previously, so they are not 16 in this packet. 17 Q. Who took these fish that were sampled? 18 A. I don't know. I wasn't there. 19 Q. Who forwarded them to your lab? 20 A. I'm not sure whether the District forwarded 21 them to our lab or whether KBM forwarded them to our 22 lab. 23 Q. Would it have to have been one or the other 24 of those? 25 A. Yes. 929 1 Q. And your lab's analysis is then contained 2 on pages -- summary on 9796 and detail on 9797 and 3 9798? 4 A. That's correct. 5 MR. SAMS: I'm going to have this whole 6 package marked as Composite Exhibit 97 so we can 7 keep trucking through here. 8 (The documents referred to were thereupon 9 marked Composite Exhibit 97 for Identification.) 10 BY MR. SAMS: 11 Q. If you could just walk us through -- 12 MR. HYDE: Did that also include 9795 or 13 was that being discarded? 14 MR. SAMS: Yes, it does. 15 THE WITNESS: With the exception of 9800, 16 which has got absolutely no reason being in 17 here. That is just a piece of paper that got 18 stuck in by mistake. I don't know where that 19 came from. 20 BY MR. SAMS: 21 Q. What belongs together next? 22 A. 9799 and 9801. 23 Q. What do they tell us? 24 A. Can we remove this page? It's really a 25 piece of paper. 930 1 MR. SAMS: Sure. Let the record reflect we 2 are removing 9800 as superfluous and unrelated. 3 THE WITNESS: These are analyses of bass 4 and catfish. 5 BY MR. SAMS: 6 Q. Where were they taken? 7 A. These were along with the KBN sample. 8 Sample HS-1-53 would tell us by looking at the chain 9 of custody form that was provided to us by KBN. 10 Q. The analyses were done in your lab? 11 A. Yes. 12 Q. And page 9801 is what? Is that an 13 averaging? 14 A. That's the summary sheet, I believe, for -- 15 that would be the averages for these sheets, I 16 believe for the top data sheet. 17 Q. Next, what belongs together? 18 A. 9802 through 9804 are some data sheets for 19 the samples. It says EPA samples collected on March 20 10, 1994. And this would be the raw data, if you 21 will, or the machine output data from -- 22 Q. How does one identify the sample sites? 23 A. Under sample name it has S-6, S-5A, S-6, 24 S-12D. S-6 would be the S-6 structure. 25 Q. These are all structures? 931 1 A. Yes. 2 Q. Where were these collected above or below 3 the structure? Can you identify -- 4 A. I believe they are collected above the 5 structure. The South Florida Water Management 6 District's staff is collecting these samples, 7 transporting them and delivering them to our 8 laboratory. 9 At this time we are just trying to collect 10 enough data to get some idea what's going through 11 these structures as it relates to mercury and, you 12 know its -- 13 Q. All right. 14 MR. HYDE: Let me ask the last sample name, 15 C 1235 R 84? 16 THE WITNESS: Yes. 17 MR. HYDE: What is that referring to? 18 THE WITNESS: That's along Alligator Alley. 19 I'm not certain. These are structures that were 20 selected or areas that were selected by the 21 District and EPA and myself. We all talked 22 about them, but I'm not sure which one that is. 23 I would just think that's 84 because of R 24 84 makes me think of Alligator Alley. That's 25 the only -- you know, that's the only thing 932 1 there. It could also be the field blank, for 2 that matter. 3 BY MR. SAMS: 4 Q. Is it your understanding, Dr. Jones, that 5 these were at sites identified for sampling as the 6 first 50 on cycle zero of E-map? 7 A. No, this is a totally different set. This 8 is a biweekly sampling for mercury at these 9 structures throughout the Everglades. 10 Q. Is that the working sample by soft mud? 11 A. By the South Florida Water Management 12 District, yes. 13 Q. What is the next set that belongs together? 14 A. I'm going to have to go back and retract 15 something I said on the first page. 9795 -- and 16 because I said Jennifer, I see now it's MEHG 17 concentration. It's not a properly labeled table. 18 It's better off later on. This is methyl mercury and 19 not total mercury. It does not have anything to do 20 with 9802. 21 The next set of documents that go together 22 would be 9805 -- I believe it goes to 9808. 23 Q. What was the sampling episode from which 24 these analyses were conducted? 25 A. It's again KBN samples that were fish 933 1 samples that were sent to us by either KBN or the 2 District. 3 Q. And the analyses, including the summary 4 tables, were prepared in your lab? 5 A. Yes. 6 Q. What's the difference between normalized 7 mercury concentration parts per billion and corrected 8 mercury parts per trillion? 9 A. The PPT value is the value for what was in 10 the sample as a liquid. And when we normalize it, we 11 normalize it to the way of the fish so that's 12 converting it into nanogram per liter, micrograms per 13 gram. 14 Q. The next set of pages that go together are 15 what? 16 A. 9809 and 9810. I don't know that there is 17 a summary table for these. 18 Q. You said 9809? 19 A. Yes, and 9810. 20 Q. And what do these analyses relate to. That 21 was again KBN samples? 22 A. Yes, they are. 23 Q. Then what? 24 A. 9811 through 9816 is another one of these 25 things that give us an idea of how many samples we 934 1 might be expecting to analyze from the EPA. 2 Q. Then we come to 9817? 3 A. Which is an individual single document. 4 Q. What does that relate to? 5 A. We were trying to get an idea of how 6 difficult it was to filter water and what kind of 7 variation we would see by removing different size 8 fractions. So we took some lake water and added some 9 soil to it, shook it up and then ran it through these 10 different sized filters, unfiltered 20 micron, 5 11 micron, 12 micron and 1 micron, to see what 12 proportion of the mercury was removed by doing the 13 filter. 14 This was more from how difficult is it to 15 filter a sample than actually what the values are for 16 mercury. But we do intend to do these now with 17 natural water, since we found out we could do it with 18 essentially mud. 19 Q. Is there any other page that goes with 20 this? 21 A. No. 22 Q. What belongs together next? 23 A. 9818 through 9820. I don't believe there 24 are any duplicates so far in what we have hit, but 25 it's possible. I haven't pulled together the R 935 1 mercury data other than to make some rough comparison 2 with KBN's. This is the same thing. This is more 3 fish samples. 4 MR. HYDE: I don't have the pages beginning 5 with 9818. 6 MR. SAMS: That turned out to be an extra. 7 I have a duplicate. 8 MS. PONZOLI: I think Dr. Jones brought 9 them to my office. 10 THE WITNESS: I put my people under some 11 pressure to produce copies. I think that's what 12 happened. 13 MR. HYDE: This is all still part of 14 composite Exhibit 97? 15